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Team:Evry/Notebook/w11
From 2013.igem.org
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| - | Top 10 <i>E. Coli</i> is transformed with the plasmid PTK which contain λ Red factor. We plated our bacteria on LB Agar with spectinomycin and let them grow overnight at 30°C. | + | Chemically competent Top 10 <i>E. Coli</i> is transformed with the plasmid PTK which contain λ Red factor. We plated our bacteria on LB Agar with spectinomycin and let them grow overnight at 30°C. One colony had grown on our plate. We started an overnight culture of this colony in 2 mL of LB with spectinomycin and still at 30°C. We then diluted 1 mL of our culture in 100 mL of LB with spectinomycin and IPTG so the λ Red factor would start to be expressed. We let bacteria grew to a OD of 0.5 and made them electro competent. |
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| + | <h3>Electro competent </h3> | ||
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Revision as of 13:23, 30 August 2013
Week 11: 26th August - 1st September
TECAN
Creation of a E. Coli ΔFur
Strain preparation
Chemically competent Top 10 E. Coli is transformed with the plasmid PTK which contain λ Red factor. We plated our bacteria on LB Agar with spectinomycin and let them grow overnight at 30°C. One colony had grown on our plate. We started an overnight culture of this colony in 2 mL of LB with spectinomycin and still at 30°C. We then diluted 1 mL of our culture in 100 mL of LB with spectinomycin and IPTG so the λ Red factor would start to be expressed. We let bacteria grew to a OD of 0.5 and made them electro competent.
Electro competent
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