Revision as of 14:28, 25 September 2013

Notebook : July 10

Abdou

Used quantities :

@@ Line 1: / Line 1: @@
-='''Notebook : July 15'''=
+{{Team:Paris_Saclay/incl_debut_generique}}
+='''Notebook : July 10'''=
 =='''Lab work'''==
-constructing
-<br>
+==='''A - Aerobic/Anaerobic regulation system'''===
+===='''Objective : obtaining Bba_K1155003, Bba_K1155007'''====
+===='''1 - Transformation of Bba_I732019 and Bba_B0010 in DH5α'''====
+==='''B - PCB sensor system'''===
+===='''Objective : obtaining Bba_K1155001, Bba_K1155002, BphR2'''====
+===='''1 - Digestion of Bba_K11001, Bba_K1155002 and BphR2 in PSB1C3 by EcoRI/PstI and SacII to check the size of fragments'''====
+Abdou
+Used quantities :
+* EcoRI/PstI :
+** DNA : 8µL
+** Buffer Orange : 1.6µL
+** EcoRI : 1µL
+** PstI : 1µL
+** H2O : 4.4µL
+* SacII :
+** DNA : 8µL
+** Buffer Blue : 1.6µL
+** SacII : 1µL
+** H2O : 5.4µL
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