"
28/08/13
From 2013.igem.org
(Difference between revisions)
(→gel electrophoresis) |
(→DNA extraction from gel) |
||
| Line 18: | Line 18: | ||
==DNA extraction from gel== | ==DNA extraction from gel== | ||
| - | *Extracting previously run DNA from gel using Thermo Scientific extraction kit and following its protocol. | + | *Extracting previously run DNA from gel using Thermo Scientific GeneJet gel extraction kit and following its protocol. |
*Concentrations of DNA were measured by nanodrop | *Concentrations of DNA were measured by nanodrop | ||
*Sample B (Tod C gene) was not purified correctly, another PCR is needed. | *Sample B (Tod C gene) was not purified correctly, another PCR is needed. | ||
Revision as of 14:01, 29 August 2013
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
|---|
Gel electrophoresis
- Two different sets of gel were run due to the different fragment size of the PCR product.
- Smaller fragments were run on a 2% agarose gel whilst larger fragments were run on a 1% gel.
DNA extraction from gel
- Extracting previously run DNA from gel using Thermo Scientific GeneJet gel extraction kit and following its protocol.
- Concentrations of DNA were measured by nanodrop
- Sample B (Tod C gene) was not purified correctly, another PCR is needed.
- Table representing the concentration and volume of DNA isolated from samples
| Sample | Volume | Concentration ng/ul | 260/280 | 260/230 |
| A | 48 | 19.3 | 2.02 | 0.03 |
| C | 50 | 27.7 | 1.88 | 0.04 |
| D | 49 | 26.9 | 1.71 | 0.05 |
| E | 50 | 20.5 | 1.98 | 0.03 |
