Team:BYU Provo/Notebook/Cholera - Enzyme/July-August/Period4/Dailylog

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Revision as of 21:26, 6 September 2013


Cholera - Enzymes Notebook: May 1 - May 14 Daily Log



Overview
March-April
May-June
July-August
September

8/21/13

Although we got a decent band for our DspB PCR product, the amount of product and the concentration was low enough that we decided to run another cycle of PCR on the product to get more product and increase the concentration of our product.

Our PCR tubes were labeled:

  1. DspB
  2. DspB Control


8/23/13

We prepared and ran our PCR product on gel, however there was a problem with the gel and the ladder bled out, so we will re-run our product on gel again.

We also replated Bacillis subtilis on an LB plate and placed it in the 37 degree incubator.