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Team:BYU Provo/Notebook/Cholera - Enzyme/September/Period1/Dailylog
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<font size="4"> '''9/4/13''' </font> | <font size="4"> '''9/4/13''' </font> | ||
| - | Today we set up phusion and Taq PCR for B. subtilis and phusion PCR for dspB -NRS | + | Today we set up phusion and Taq PCR for B. subtilis and phusion PCR for dspB |
| + | |||
| + | Phusion PCR setup | ||
| + | 35 microliters ddH2O | ||
| + | 10 microliters 5X phusion buffer | ||
| + | 1.5 microliters 10mM dNTP's | ||
| + | 1 microliter each primer | ||
| + | 1 microliter diluted template DNA | ||
| + | .5 microliter phusion polymerase | ||
| + | |||
| + | Taq PCR | ||
| + | 40 microliters ddH2O | ||
| + | 5 microliters 10X TAQ buffer | ||
| + | 1.5 microliters 10mM dNTP's | ||
| + | 1 microliter each primer | ||
| + | 1 microliter diluted template DNA | ||
| + | .5 microliter phusion polymerase | ||
| + | -NRS | ||
<br> | <br> | ||
Revision as of 22:53, 6 September 2013
| Cholera - Enzymes Notebook: September 1 - September 14 Daily Log
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9/4/13 Today we set up phusion and Taq PCR for B. subtilis and phusion PCR for dspB Phusion PCR setup 35 microliters ddH2O 10 microliters 5X phusion buffer 1.5 microliters 10mM dNTP's 1 microliter each primer 1 microliter diluted template DNA .5 microliter phusion polymerase Taq PCR 40 microliters ddH2O 5 microliters 10X TAQ buffer 1.5 microliters 10mM dNTP's 1 microliter each primer 1 microliter diluted template DNA .5 microliter phusion polymerase -NRS
9/5/2013 I ran the PCR products from 9/4 on gel and there were no bands. -NRS
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