Revision as of 20:42, 10 September 2013

Notebook : July 3

Continued what we started yesterday. Observed the Petri dish, selected the colonies. 4 colonies in total, they were 2 include FNR+plasmid in PSB1C3 and 2 from the control.
Used 4 primers: VF2, VR, Pfnr_up, Pfnr_down for the verification test. They were designed to cut 4 special sites for creating 3 different regions on plasmid chain: VF2/VR, VF2/PFNR_down, PFNR_up/VR. After the amplification, those PCR products had been put on electrophoresis gel for the verification.

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 =='''Summary:'''==
-*Observed the Petri dish, we decided to perform a PCR test for the colonies from these 4 mediums.
-*From those incubated plates, 4 colonies (2 from FNR+plasmid PSB1C3, 2 from LacZ and AmilCP) were selected in parallel for further test.
+*Continued what we started yesterday. Observed the Petri dish, selected the colonies. 4 colonies in total, they were 2 include FNR+plasmid in PSB1C3 and 2 from the control.
-*Used 4 primers: VF2, VR, PFNR_up, PFNR_down for verification. They were designed to cut 4 special sites for creating 3 different regions on plasmid chain: VF2/VR, VF2/PFNR_down, PFNR_up/VR. The PCR products had been put on electrophoresis gel for the verification
+*Used 4 primers: VF2, VR, Pfnr_up, Pfnr_down for the verification test. They were designed to cut 4 special sites for creating 3 different regions on plasmid chain: VF2/VR, VF2/PFNR_down, PFNR_up/VR. After the amplification, those PCR products had been put on electrophoresis gel for the verification.
 =='''Lab work'''==