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Team:Paris Saclay/Notebook/August/19
From 2013.igem.org
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==='''A - Aerobic/Anaerobic regulation system / B - PCB sensor system'''=== | ==='''A - Aerobic/Anaerobic regulation system / B - PCB sensor system'''=== | ||
| - | ===='''Objective : | + | ===='''Objective : obtaining FNR and BphR2 proteins (Gibson assembly) '''==== |
| + | |||
| + | =====1 - Electrophoresis gel of PCR products : BphR2 Part I===== | ||
| - | |||
Damir | Damir | ||
{| | {| | ||
| - | | style="width:250px;border:1px solid black;" | [[ | + | | style="width:250px;border:1px solid black;" | [[]] |
| style="width:350px;border:1px solid black;vertical-align:top;" | | | style="width:350px;border:1px solid black;vertical-align:top;" | | ||
*Well 1 : 6µL DNA Ladder | *Well 1 : 6µL DNA Ladder | ||
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{| | {| | ||
| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" | | | style="border:1px solid black;padding:5px;background-color:#DEDEDE;" | | ||
| - | We obtain a frangment at the | + | We obtain a frangment at the right size. We can purify it. |
|} | |} | ||
| - | =====2 - Gel purification of BphR2 ===== | + | =====2 - Gel purification of PCR products : BphR2 Part I ===== |
| + | |||
Damir | Damir | ||
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* BphR2 : 57.2ng/µL | * BphR2 : 57.2ng/µL | ||
| - | CONCLUSION | + | {| |
| + | | style="border:1px solid black;padding:5px;background-color:#DEDEDE;" | | ||
| + | CONCLUSION | ||
| + | |} | ||
=='''Meeting with Evry'''== | =='''Meeting with Evry'''== | ||
Revision as of 22:36, 10 September 2013
Contents |
Notebook : August 19
Lab work
A - Aerobic/Anaerobic regulation system
Objective : to obtain ...
1 - Gel purification of the digestion of Bba_K1155003 and Bba_K1155007 by XBaI/PstI
Nadia
Protocol : Gel purification
Nanodrop :
- RBS-LacZ-Term : 59.6ng/µL
- RBS-AmilCP-Term : 21.3ng/µL
|
Concentrations are very low so we try to precipitate the DNA again. |
2 - EtOH precipitation of RBS-LacZ-Term and RBS-AmilCP-Term
Nadia, XiaoJing
Protocol : EtOH precipitation Protocole ou à rédiger ici ?????????
We used 20µL of DNA.
Nanodrop :
- RBS-LacZ-Term : 38.4ng/µL
- RBS-AmilCP-Term : 28.7ng/µL
|
CONCLUSION |
A - Aerobic/Anaerobic regulation system / B - PCB sensor system
Objective : obtaining FNR and BphR2 proteins (Gibson assembly)
1 - Electrophoresis gel of PCR products : BphR2 Part I
Damir
| [[]] |
|
Expected size :
- BphR2 Part I : 178 kb
|
We obtain a frangment at the right size. We can purify it. |
2 - Gel purification of PCR products : BphR2 Part I
Damir
Protocol : Gel purification
Nanodrop :
- BphR2 : 57.2ng/µL
|
CONCLUSION |
Meeting with Evry
