Team:Paris Saclay/Notebook/August/22

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=='''Lab work'''==
=='''Lab work'''==
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==='''A - Aerobic/Anaerobic regulation system'''===
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===='''Objective : obtaining ...'''====
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===='''1 - Plasmid extraction of Bba_K1155000 from ...'''====
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Nguyen
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Protocol : [[Team:Paris_Saclay/Protocols/plasmid extraction|Plasmid extraction]]
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Nanodrop :
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* Bba_K1155000 : 175ng/µL
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{|
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| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" |
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CONCLUSION !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
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|}
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===='''2 - Digestion of Bba_K1155000 by SpeI'''====
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Nguyen
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Used quantities :
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* Bba_K1155000 : 10µL
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* Buffer FD : 2µL
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* Spe I : 2µL
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* H2O : 6 µL
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QU'EN A T'ON FAIT PAR LA SUITE ?????????
==='''A - Aerobic/Anaerobic regulation system / B - PCB sensor system'''===
==='''A - Aerobic/Anaerobic regulation system / B - PCB sensor system'''===

Revision as of 21:48, 11 September 2013

Navigation Home Team Project Labwork Notebook Human practices

Contents

Notebook : August 22

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining ...

1 - Plasmid extraction of Bba_K1155000 from ...

Nguyen

Protocol : Plasmid extraction

Nanodrop :

  • Bba_K1155000 : 175ng/µL

CONCLUSION !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!

2 - Digestion of Bba_K1155000 by SpeI

Nguyen

Used quantities :

  • Bba_K1155000 : 10µL
  • Buffer FD : 2µL
  • Spe I : 2µL
  • H2O : 6 µL

QU'EN A T'ON FAIT PAR LA SUITE ?????????

A - Aerobic/Anaerobic regulation system / B - PCB sensor system

Objective : obtaining BphR2 protein

1 - Electrophoresis of PCR product : BphR2 Part I

Damir

]]
  • Well 1 : 6µL DNA Ladder
  • Well 2 : 40µL of BphR2 Part I+8µl of 6X loading dye
  • Gel : 0.8%

Expected sizes : Bphr2 Part I : 178 kb

We obtain fragments at the right size. We can purify it.

2 - Gel purification of PCR product : BphR2 Part I

Damir

Protocol : Gel purification

Nanodrop :

  • RBS-BphR2 Part I, tube 1 : 42ng/µL
  • RBS-BphR2 Part I, tube 2 : 75ng/µL

CONCLUSION !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!

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