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Team:Paris Saclay/Notebook/August/22
From 2013.igem.org
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QU'EN A T'ON FAIT PAR LA SUITE ????????? | QU'EN A T'ON FAIT PAR LA SUITE ????????? | ||
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| + | ===='''Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in PSB3K3'''==== | ||
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| + | ===='''1 - Gel purification of the digestion of Bba_J04450 by EcoRI/PstI ''==== | ||
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| + | XiaoJing | ||
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| + | Protocol : [[Team:Paris_Saclay/Protocols/Gel purification|Gel purification]] | ||
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| + | Nanodrop : | ||
| + | * PSB3K3 : 4ng/µL | ||
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==='''A - Aerobic/Anaerobic regulation system / B - PCB sensor system'''=== | ==='''A - Aerobic/Anaerobic regulation system / B - PCB sensor system'''=== | ||
Revision as of 21:57, 11 September 2013
Notebook : August 22
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining ...
1 - Plasmid extraction of Bba_K1155000 from ...
Nguyen
Protocol : Plasmid extraction
Nanodrop :
- Bba_K1155000 : 175ng/µL
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CONCLUSION !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! |
2 - Digestion of Bba_K1155000 by SpeI
Nguyen
Used quantities :
- Bba_K1155000 : 10µL
- Buffer FD : 2µL
- Spe I : 2µL
- H2O : 6 µL
QU'EN A T'ON FAIT PAR LA SUITE ?????????
Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in PSB3K3
'1 - Gel purification of the digestion of Bba_J04450 by EcoRI/PstI
XiaoJing
Protocol : Gel purification
Nanodrop :
- PSB3K3 : 4ng/µL
A - Aerobic/Anaerobic regulation system / B - PCB sensor system
Objective : obtaining BphR2 protein
1 - Electrophoresis of PCR product : BphR2 Part I
Damir
| ]] |
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Expected sizes : Bphr2 Part I : 178 kb
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We obtain fragments at the right size. We can purify it. |
2 - Gel purification of PCR product : BphR2 Part I
Damir
Protocol : Gel purification
Nanodrop :
- RBS-BphR2 Part I, tube 1 : 42ng/µL
- RBS-BphR2 Part I, tube 2 : 75ng/µL
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CONCLUSION !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! |
