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Team:Paris Saclay/Notebook/August/2
From 2013.igem.org
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| - | We obtain fragment at the right size. ON A LA BIOBRICK ?????????? | + | We obtain fragment at the right size (Clone 12 starts 5 min after the others). ON A LA BIOBRICK ?????????? |
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| + | ===='''Objective : obtaining Bba_K1155004, Bba_K1155005, Bba_K1155006'''==== | ||
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| + | ==='''A - Aerobic/Anaerobic regulation system / B - PCB sensor system'''=== | ||
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| + | ===='''Objective : obtaining FNR and BphR2 proteins'''==== | ||
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| + | ===='''1 - Tranformation of BphR2, FNR and RBS-FNR in DH5α'''==== | ||
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| + | XiaoJing | ||
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| + | Protocol : [[Team:Paris_Saclay/Protocols/Bacterial transformation|Bacterial transformation]] | ||
{{Team:Paris_Saclay/incl_fin}} | {{Team:Paris_Saclay/incl_fin}} | ||
Revision as of 22:14, 15 September 2013
Contents |
Notebook : August 1
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Bba_K1155003
1 - Extraction of Bba_K1155003 from DH5α
Damir, Nadia
Protocol : Plasmid extraction
We used colony 9, 11 and 12. ???????? Elué dans 50µL d'H2O.
2 - Electrophoresis of the extraction of Bba_K1155003
Damir, Nadia
| File:Ps.jpg|350px]] |
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Expected sizes :
- Bba_K1155003 : 2734 pb
Estimated concentrations :
- Clone 9 : 18ng/µL
- Clone 11 :18ng/µL
- Clone 12 : 18ng/µL
|
We obtain fragment at the right size (Clone 12 starts 5 min after the others). ON A LA BIOBRICK ?????????? |
Objective : obtaining Bba_K1155004, Bba_K1155005, Bba_K1155006
A - Aerobic/Anaerobic regulation system / B - PCB sensor system
Objective : obtaining FNR and BphR2 proteins
1 - Tranformation of BphR2, FNR and RBS-FNR in DH5α
XiaoJing
Protocol : Bacterial transformation
