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Team:Paris Saclay/Notebook/August/5
From 2013.igem.org
(Difference between revisions)
| Line 31: | Line 31: | ||
* Well 1 : | * Well 1 : | ||
* Well 2 : 6µL of DNA Ladder | * Well 2 : 6µL of DNA Ladder | ||
| - | * Well 3 : 30µL of Bba_K1155003 digested by EcoRI/PstI+6µl of 6X loading dye | + | * Well 3 : 30µL of Bba_K1155003 from clone 9 digested by EcoRI/PstI+6µl of 6X loading dye |
| - | * Well 4 : | + | * Well 4 : 5µL of Bba_K1155003 from clone 9+1µl of 6X loading dye |
| - | * Well 5 : | + | * Well 5 : 30µL of Bba_K1155003 from clone 11 digested by EcoRI/PstI+6µl of 6X loading dye |
| - | * Well 6 : | + | * Well 6 : 5µL of Bba_K1155003 from clone 11+1µl of 6X loading dye |
| - | * Well 7 : | + | * Well 7 : 30µL of Bba_K1155003 from clone 12 digested by EcoRI/PstI+6µl of 6X loading dye |
| - | * Well 8 : | + | * Well 8 : 5µL of Bba_K1155003 from clone 12+1µl of 6X loading dye |
* Gel : 1% | * Gel : 1% | ||
|} | |} | ||
{{Team:Paris_Saclay/incl_fin}} | {{Team:Paris_Saclay/incl_fin}} | ||
Revision as of 22:42, 15 September 2013
Contents |
Notebook : August 5
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Bba_K1155003
1 - Digestion of Bba_K1155003 by EcoRI/PstI
Nadia, XiaoJing
Used quantities :
- Bba_K1155003 : 5µL
- EcoRI FD : 1µL
- PstI FD : 1µL
- Buffer FD : 3µL
- H2O : 20µL
We let the digestion at 37°C during 10 minutes ??????
2 - Electrophoresis of the digestion of Bba_K1155003
Damir
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