Revision as of 16:15, 16 September 2013

Notebook : August 6

XiaoJing, Damir, Anaïs

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[[]]	Well 1 to 12 : 10µL Bba_K1155006+2µl of 6X loading dye Well 13 : 6µL DNA Ladder Well 14 to 26 : 10µL Bba_K1155006+2µl of 6X loading dye Gel : 1%

Expected size :

We obtain fragments at the good size for all the colony. ?????????

Xiaoing, Anaïs

Used quantities :

We let the incubation over night at 37°C at 180 RPM.

We used colonies number 6, 7 and 8 for each promotor.

IMAGE LOGICIEL

The sequence is good for each clone. We obtain a new biobrick : Bba_K1155002.

@@ Line 65: / Line 65: @@
 {|
 | style="border:1px solid black;padding:5px;background-color:#DEDEDE;" |
-We obtain fragments at the good size for all the colony. ENSUITE ?????????
+We obtain fragments at the good size for all the colony.  ?????????
 |}
-====2 - Samples culture====
+====2 - Culture of DH5α with Bba_K1155004, Bba_K1155005 and Bba_K1155006 ====
 Xiaoing, Anaïs
-We put in culture the 6, 7, 8 samples for every promoter in 5 ml LB + 5 µl Chlorenphenicol (1000x,20µg/ml)
+Used quantities :
-Incubator over night to 310,15K at 180 RPM
+* LB : 5mL
+* Chloramphénicol (1000X, 20µg/mL) : 5µL
+* Bba_K1155004, Bba_K1155005 and Bba_K1155006 : 25µL ?????????
-==='''B - PCB sensing system'''===
+We let the incubation over night at 37°C at 180 RPM.
-===='''Obtaining the BphA1 promoter'''====
+We used colonies number 6, 7 and 8 for each promotor.
-====1 - Sequence analysis for BB pBphA1 in psB1C3 5 ( clone 4, 17 and 22 )====
-IMAGE
-==='''A - Aerobic/Anaerobic regulation system / B - PCB sensing system'''===
-====1 - PCR product( made the 08/01/2013)digestion to degrade the backbone psB1C3====
+==='''B - PCB sensing system'''===
+===='''Objective : obtaining Bba_K1155002'''====
+====1 - Sequence analysis for Bba_K1155002 in clones 4, 17 and 22====
+IMAGE LOGICIEL
+{|
+| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" |
+The sequence is good for each clone. We obtain a new biobrick : Bba_K1155002.
+|}
 {{Team:Paris_Saclay/incl_fin}}