Team:Paris Saclay/PS-PCR/detailed description

From 2013.igem.org

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The Paris-Saclay open source PCR thermal cycler project.
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== Motivation ==
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PCR (Polymerase Chain Reaction) is a very useful procedure in the field of synthetic biology. However, this technique requires a device called a ''thermal cycler'', which is designed to cycle the samples through different temperature steps. Commercial PCR thermal cyclers are traditionally very expensive, with prices starting at $2000, which makes them hard to afford for small labs and DIY projects.
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This project aims at creating a thermal cycler for under 30€ with easily accessible parts.
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=== Comparison with other open source projects ===
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Some open source PCR thermal cycler projects already exist, but the aim of the PSPCR project is to provide a better and more affordable system.
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{| class="wikitable"
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|-
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! scope="col"| Project
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! scope="col"| Price
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! scope="col"| Discussion
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|-
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! scope="row"| [http://openpcr.org/ OpenPCR]
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| $649
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| Heated lid, good performance but still expensive.
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|-
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! scope="row"| [http://www.instructables.com/id/Arduino-PCR-thermal-cycler-for-under-85/ Arduino PCR]
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| $85
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| No heated lid, room for only 2 tubes, fan-only cooling.
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|-
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! scope="row"| [http://lavaamp.wordpress.com/ LavaAmp]
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| "pennies"
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| No data available, probably slow with no heated lid.
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|-
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! scope="row" style="background-color:#DEDEDE;"| PSPCR
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| Under 30€
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| Fast heating/cooling, 16 slots, heated lid.
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|-
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|}
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{{Team:Paris_Saclay/incl_fin}}

Revision as of 21:56, 16 September 2013

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PSPCR

The Paris-Saclay open source PCR thermal cycler project.

Motivation

PCR (Polymerase Chain Reaction) is a very useful procedure in the field of synthetic biology. However, this technique requires a device called a thermal cycler, which is designed to cycle the samples through different temperature steps. Commercial PCR thermal cyclers are traditionally very expensive, with prices starting at $2000, which makes them hard to afford for small labs and DIY projects.

This project aims at creating a thermal cycler for under 30€ with easily accessible parts.

Comparison with other open source projects

Some open source PCR thermal cycler projects already exist, but the aim of the PSPCR project is to provide a better and more affordable system.

Project Price Discussion
[http://openpcr.org/ OpenPCR] $649 Heated lid, good performance but still expensive.
[http://www.instructables.com/id/Arduino-PCR-thermal-cycler-for-under-85/ Arduino PCR] $85 No heated lid, room for only 2 tubes, fan-only cooling.
[http://lavaamp.wordpress.com/ LavaAmp] "pennies" No data available, probably slow with no heated lid.
PSPCR Under 30€ Fast heating/cooling, 16 slots, heated lid.

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