"
Team:Paris Saclay/Notebook/July/12
From 2013.igem.org
(Difference between revisions)
(→Lab work) |
(→Notebook : July 12) |
||
| Line 15: | Line 15: | ||
<br> | <br> | ||
| + | <u>Transformation</u> | ||
| + | <br> | ||
| + | <p>We suspended the DNA with water from iGEM plate and performed several transformation.</p><br> | ||
| + | *BBa_I732019: LacZ+RBS+terminator into plasmid BBa_I732950(ampicillin resitant). | ||
| + | *BBa_B0015: 2 terminators BBa_B0010+BBa_B0012(4F plate 3 kit 2013) into plasmid PSB1C3. | ||
| + | *BBa_B0017: 2 terminators BBa_B0010+BBa_B0010 into plasmid PSB1C3. | ||
| + | *Simple terminator BBa_B0010 into plasmid PSB1A2. | ||
| + | |||
| + | see protocol Tranformation. | ||
| + | |||
| + | B.PCBs sensor system: | ||
| + | <p>The stock and extraction of promoter BphR1 clone 5 and 6, promoter BphR2 clone 3 and 4, promoter BphA1 clone 5 to 8 performed according to protocol. Please see protocol DNA extraction and sample stock.</p> | ||
<br> | <br> | ||
Revision as of 02:26, 22 September 2013
Notebook : July 12
summary
For fnr regulator system:
- Performed 4 transformations(3 terminator and 1 RBS+LacZ+terminator into competent cells).
- Design of oligo for amplifying AmiCP+RBS.
For PCBs sensor system:
- Had the promoter BphR1 clone 5 and 6, promoter BphR2 clone 3 and 4, promoter BphA1 clone 5 to 8 in stock.
- Plasmid DNA extraction from promoter BphR1 clone 5 and 6, promoter BphR2 clone 3 and 4, promoter BphA1 clone 5 to 8.
Lab work
A.aero/anaerobic regulation system:
- BioBrick RBS+LacZ+terminator in plasmid PSB1C3
Transformation
We suspended the DNA with water from iGEM plate and performed several transformation.
- BBa_I732019: LacZ+RBS+terminator into plasmid BBa_I732950(ampicillin resitant).
- BBa_B0015: 2 terminators BBa_B0010+BBa_B0012(4F plate 3 kit 2013) into plasmid PSB1C3.
- BBa_B0017: 2 terminators BBa_B0010+BBa_B0010 into plasmid PSB1C3.
- Simple terminator BBa_B0010 into plasmid PSB1A2.
see protocol Tranformation.
B.PCBs sensor system:
The stock and extraction of promoter BphR1 clone 5 and 6, promoter BphR2 clone 3 and 4, promoter BphA1 clone 5 to 8 performed according to protocol. Please see protocol DNA extraction and sample stock.
| Previous day | Back to calendar | Next week |
