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Team:Paris Saclay/gel
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(Created page with "{{Team:Paris_Saclay/incl_debut_generique}} = '''Electrophoresis on gel''' = Gels are made with agarose in pounder with TAE 0.5X To make 1% gel: 1. Put 1g of agarose in pounder ...")
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(Created page with "{{Team:Paris_Saclay/incl_debut_generique}} = '''Electrophoresis on gel''' = Gels are made with agarose in pounder with TAE 0.5X To make 1% gel: 1. Put 1g of agarose in pounder ...")
Newer edit →
Revision as of 20:12, 22 September 2013
Electrophoresis on gel
Gels are made with agarose in pounder with TAE 0.5X To make 1% gel: 1. Put 1g of agarose in pounder 2. Add TAE until 100mL 3. Boil until the solution become uniform 4. Add BET with a final concentration of 200ng/µL 5. Pour gels in molds 6. Let agarose in suffusion at 60°C
Put the misted DNA with charged blue diluted 6X
