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Team:Paris Saclay/gibson
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Revision as of 20:14, 22 September 2013
Gibson Assembly
The Gibson assembly binds several DNA fragments with complementary ends. To realize a Gibson Assembly, we need put together 3 enzymes with different functions: an exo-nucléase in 5’, a polymerase and a ligase.
1. Put in a tube 15µL of mix Gibson
2. Add DNA fragments in molar relation
3. Add water until 20µL
4. Incubate at 50°C during 1h30 in PCR
