Team:Paris Saclay/ethanol
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(Created page with "{{Team:Paris_Saclay/incl_debut_generique}} = '''Ethanol precipitation''' = This method purifies and gathers DNA in a mixing Add in a volume of DNA : 1. 3 volumes of ethanol 10...")
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Revision as of 20:17, 22 September 2013
Ethanol precipitation
This method purifies and gathers DNA in a mixing Add in a volume of DNA :
1. 3 volumes of ethanol 100%
2. 1/10 of volume of sodium acetate 3M
3. 1µL of glycogen
4. Let the solution at -20°C during 30min
5. Centrifuge at maximal speed during 30min at 4°C
6. Put away the supernatant
7. Wash with 1mL of ethanol 70%
8. Centrifuge 10-15 min at 4°C
9. Put away the supernatant, dry (speed vacuum 4 min)
10. Repeat with water or TE