Team:British Columbia/Notebook/Protocols

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==Protocols==
==Protocols==
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===[[Team:British_Columbia/Notebook/Protocols/RepeatSpacerAssembly |Repeat-Spacer Array Assembly]]===
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===[[Team:British_Columbia/Notebook/Protocols/RepeatSpacerAssembly |CRISPR Repeat-Spacer Array Assembly]]===
Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides
Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides

Revision as of 01:04, 23 September 2013

iGEM Home

Contents

Protocols

CRISPR Repeat-Spacer Array Assembly

Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides

Assembling Parts from Oligonucleotides

Assemble short parts from annealed oligonucleotides

(Multi) Site Directed Mutagenesis

Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations.

Oligonucleotide Phosphorylation

Phosphorylate oligonucleotides prior to ligation