Team:British Columbia/Notebook/Protocols
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==Protocols== | ==Protocols== | ||
- | ===[[Team:British_Columbia/Notebook/Protocols/RepeatSpacerAssembly |Repeat-Spacer Array Assembly]]=== | + | ===[[Team:British_Columbia/Notebook/Protocols/RepeatSpacerAssembly |CRISPR Repeat-Spacer Array Assembly]]=== |
Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides | Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides | ||
Revision as of 01:04, 23 September 2013
iGEM Home
Contents |
Protocols
CRISPR Repeat-Spacer Array Assembly
Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides
Assembling Parts from Oligonucleotides
Assemble short parts from annealed oligonucleotides
(Multi) Site Directed Mutagenesis
Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations.
Oligonucleotide Phosphorylation
Phosphorylate oligonucleotides prior to ligation