Team:British Columbia/Notebook/Protocols

(Difference between revisions)

Revision as of 01:12, 23 September 2013

Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides

Assemble short parts from annealed oligonucleotides

Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations.

Phosphorylate oligonucleotides prior to ligation

Electrocompetent cells production.

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	===[[Team:British_Columbia/Notebook/Protocols/ElectrocompCells\|Electrocompetent Cell Production]]===		===[[Team:British_Columbia/Notebook/Protocols/ElectrocompCells\|Electrocompetent Cell Production]]===
-	~~Make electrocompetent~~ cells.	+	Electrocompetent cells production.