Team:Imperial College/data
From 2013.igem.org
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[[File:LB_M9.png|thumbnail|left|500px|<b>Figure 1: The medias used for our growth assays using the MG1655 strain.</b> phaCAB is an operon involved in synthesis of P3HB while EV represents an empty vector. SR refers to stress response cells containing a red pigment. M9M is minimal media, while M9S is supplemented minimal media, as described [https://2013.igem.org/Team:Imperial_College/Protocols#M9_minimal_and_supplemented_media here]. WCM refers to waste conditioned media, which is made from sterile filtrated SRF waste, see [https://2013.igem.org/Team:Imperial_College/Protocols#Waste_Conditioned_Media_.28WCM.29 here]. Error bars are SEM, n=4.]] | [[File:LB_M9.png|thumbnail|left|500px|<b>Figure 1: The medias used for our growth assays using the MG1655 strain.</b> phaCAB is an operon involved in synthesis of P3HB while EV represents an empty vector. SR refers to stress response cells containing a red pigment. M9M is minimal media, while M9S is supplemented minimal media, as described [https://2013.igem.org/Team:Imperial_College/Protocols#M9_minimal_and_supplemented_media here]. WCM refers to waste conditioned media, which is made from sterile filtrated SRF waste, see [https://2013.igem.org/Team:Imperial_College/Protocols#Waste_Conditioned_Media_.28WCM.29 here]. Error bars are SEM, n=4.]] | ||
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<h2>Waste and waste conditioned media growth assays</h2> | <h2>Waste and waste conditioned media growth assays</h2> | ||
Revision as of 11:01, 23 September 2013
Contents
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Media characterisation
Growth assays with different experimental media
Waste and waste conditioned media growth assays
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Biobrick characterisation
phaCAB biobrick characterisation
LB
M9 Minimal
M9 Supplemented
PUR enzyme characterisation
Stress biosensor characterisation
Waste Conditioned Media
pBAD characterisation
Glucose
ANOVA analysis shows that...
Plastic Toxicity Assays
L-lactic Acid
Ethylene glycol
3-hydroxybutyrate (3HB)
Acetoacetate
Poly(3-hydroxybutyrate) P(3HB)
Poly(lactic acid) (PLA)
IPTG induction assay
Originally we intended on using [http://parts.igem.org/Part:BBa_K639003 BBa_K639003] to detect whether our cells were stressed when placed in various toxic byproducts. However, as the data below shows, this biobrick is very leaky. As such, we are using the stress sensor as a marker for cell growth and also to show that the cells had been successfully transformed with the correct chloramphenicol resistance.
Sole carbon source
3HB
Acetoacetate
Induction
Empty Vector Control
Western blots
Enzyme Kinetics
PHB production