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Team:TU-Eindhoven/Chasis
From 2013.igem.org
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''Section under construction. Sorry for the inconveniences, please come back again some other time.'' | ''Section under construction. Sorry for the inconveniences, please come back again some other time.'' | ||
| - | <!-- Our device is based on Escherichia coli B strains. The current construct is observed on the figure below. The His Tag,thrombine, cleavage site and the terminator sequence optimize transcription and ultimately expression. | + | <!-- Our device is based on Escherichia coli B strains. As observed on figure 1 it comprises a series of elements that allow the detection of the tumor region due to an environmental sensor based on oxygen sensing. The device is able to reach the hypoxic region due to the flagella that the E. coli possesses, which in figure 1 are depicted as the propeller. The protein production on figure 1 refers to the CEST proteins that are generated by the bacteria when it detects the hypoxic environment. The light bulb depicted on this graphic representation illustrates the ability of the bacteria to be detected by MRI. Furthermore, the kill switch and the human cell disguise are two elements that the device should include in order to be used on the two applications we proposed, however at the current stage of the project these elements are not part of the device. |
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| + | {{:Team:TU-Eindhoven/Template:Float | position=right | size=8 }} | ||
| + | {{:Team:TU-Eindhoven/Template:Image | filename=constructDevice.png}} | ||
| + | {{:Team:TU-Eindhoven/Template:FloatEnd | caption=Graphic description of CEST protein production device.| id=graphVersionDeviceFigure }} | ||
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| + | The current construct is observed on the figure below. The His Tag,thrombine, cleavage site and the terminator sequence optimize transcription and ultimately expression. | ||
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| + | {{:Team:TU-Eindhoven/Template:Float | position=right | size=8 }} | ||
| + | {{:Team:TU-Eindhoven/Template:Image | filename=constructDevice.png}} | ||
| + | {{:Team:TU-Eindhoven/Template:FloatEnd | caption=Current construct of the CEST protein producing device.| id=constructFigure }} | ||
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| + | In the future, the chassis should contain : | ||
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| + | * Kill switch vector | ||
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| + | * Human cell disguise vector | ||
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| + | The kill switch vector is constituted by the prodrug Ganciclovir/Herpes Simplex Virus Thymidine Kinase Enzyme system. Including this vector on our device will allow the termination of the bacterial device at any point of the CEST MRI process. This system is explained on detail in the [[Team:TU-Eindhoven/KillingMechanism| kill switch section of the project tab.]] | ||
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| + | The human cell disguise refers to the alteration that should be induced in the external layer of the bacteria in order to become invisible to the immune system and avoid causing an immune system response. The detailed change that E. coli should endured in described on the [[ Team:TU-Eindhoven/Applications | application section of the project tab]]. | ||
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Revision as of 22:33, 23 September 2013
Section under construction. Sorry for the inconveniences, please come back again some other time.
