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Team:Paris Saclay/Labwork
From 2013.igem.org
(Difference between revisions)
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* Denaturation of EcoRI/SpeI used of the digestion of PCR products : NirB | * Denaturation of EcoRI/SpeI used of the digestion of PCR products : NirB | ||
| + | |||
| + | 07/31/13 | ||
| + | * Ligation of NirB in PSB1C3 | ||
| + | * Electrophoresis to check the ligation of NirB in PSB1C3 | ||
| + | * Transformation of Bba_K1155004 | ||
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* Denaturation of EcoRI/SpeI used of the digestion of PCR products : NarG | * Denaturation of EcoRI/SpeI used of the digestion of PCR products : NarG | ||
| + | |||
| + | 07/31/13 | ||
| + | * Ligation of NarG in PSB1C3 | ||
| + | * Electrophoresis to check the ligation of NarG in PSB1C3 | ||
| + | * Transformation of Bba_K1155005 | ||
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* Denaturation of EcoRI/SpeI used of the digestion of PCR products : NarK | * Denaturation of EcoRI/SpeI used of the digestion of PCR products : NarK | ||
| + | |||
| + | 07/31/13 | ||
| + | * Ligation of NarK in PSB1C3 | ||
| + | * Electrophoresis to check the ligation of NarK in PSB1C3 | ||
| + | * Transformation of Bba_K1155006 | ||
| Line 298: | Line 313: | ||
* Electrophoresis to check the digestion of Bba_I732017 by EcoRI/SpeI [File:Psright.png|20px]] | * Electrophoresis to check the digestion of Bba_I732017 by EcoRI/SpeI [File:Psright.png|20px]] | ||
* Gel purification of digestion of Bba_I732017 by EcoRI/PstI [[File:Pswrong.png|20px]] | * Gel purification of digestion of Bba_I732017 by EcoRI/PstI [[File:Pswrong.png|20px]] | ||
| + | |||
| + | 07/31/13 | ||
| + | * Digestion of Bba_I732017 by EcoRI/SpeI | ||
| + | * Electrophoresis to check the digestion of Bba_I732017 by EcoRI/SpeI [File:Psright.png|20px]] | ||
| + | * Gel purification of digestion of RBS-LacZ [[File:Pswrong.png|20px]] | ||
Revision as of 00:04, 27 September 2013
Contents |
Labwork overview
Bba_K1155000
07/01/13
- Digestion of PSB1C3 plasmid and PCR products : Pfnr by EcoRI/ PstI
- Ligation of PSB1C3 and Pfnr
07/02/13
- Tranformation of Bba_K1155000 in DH5α
07/03/13
- Colony PCR of Bba_K1155000 to check good insertion of Pfnr in PSB1C3
- New culture of Bba_K1155000
07/04/13
- Estimation of Pfnr Colony PCR size fragments thanks to Gene manager
- Electrophoresis of Colony PCR products
- Stock of Bba_K1155000
- Culture of Bba_K1155000
- Extraction of Bba_K1155000 from DH5α
- Digestion of Bba_K1155000 by NotI, MluI, HpaI to check good insertion of Pfnr in PSB1C3 again
07/05/13
- Electrophoresis of the digestion of Bba_K1155000 by NotI, HpaI, MluI
- Extraction of Bba_K1155000 from DH5α
07/15/13
- Sequensis analysis of Bba_K1155000
WE OBTAIN OUR BIOBRICK.
Bba_K1155001
07/09/13
- PCR purification of BphR1
- Digestion PCR products : BphR1 and digestion of PSB1C3
- Denaturation of EcoRI/PstI used for the digestion of PCR products : BphR1 and PSB1C3
- Ligation of BphR1 and PSB1C3
07/10/13
- Transformation of ligation of PSB1C3 and BphR1
07/11/13
- Colony PCR of Bba_K1155001 in DH5α to check good insertion of BphR1 in PSB1C3
- Electrophoresis of Colony PCR products : Bba_K1155001 in PSB1C3
07/12/13
- Stock of clones 5, 6 for Bba_K1155001
- Extraction of clones 5, 6 for Bba_K1155001 from DH5α
07/15/13
- Digestion of Bba_K1155001 by EcoRI/PstI and SacII to check the size of fragments
- PCR of digestion of Bba_K1155001
07/16/13
- Electrophoresis of the digestion of Bba_K1155001
07/17/13
- Stock of Bba_K1155001
- Extraction of Bba_K1155001 from DH5α
07/24/13
- Sequence analysis of Bba_K1155001
WE OBTAIN OUR BIOBRICK.
Bba_K1155002
07/09/13
- PCR purification of BphA1
- Digestion PCR products : BphA1 and digestion of PSB1C3
- Denaturation of EcoRI/PstI used for the digestion of PCR products : BphA1 and PSB1C3
- Ligation of BphA1 and PSB1C3
07/10/13
- Transformation of ligation of PSB1C3 and BphA1
07/11/13
- Colony PCR of Bba_K1155002 in DH5α to check good insertion of BphA1 in PSB1C3
- Electrophoresis of Colony PCR products : Bba_K1155002 in PSB1C3
07/12/13
- Stock of clones 5, 6, 7, 8 for Bba_K1155002
- Streak of Bba_K1155002
- Extraction of clones 5, 6, 7, 8 for Bba_K1155002 from DH5α
07/15/13
- Digestion of Bba_K1155002 by EcoRI/PstI and SacII to check the size of fragments
- PCR of digestion of Bba_K1155002
07/16/13
- Electrophoresis of the digestion of Bba_K1155002
07/25/13
- Ligation of BphA1 in PSB1C3
07/26/13
- Tranformation of Bba_K1155002 in DH5α
07/30/13
- Colony PCR of Bba_K1155002 in DH5α
- Electrophoresis of the Colony PCR of Bba_K1155002 in DH5α
Bba_K1155003
07/02/13
- Tranformation of Bba_K592009 in DH5α
07/03/13
- New culture of Bba_K592009
07/05/13
- Extraction of Bba_K592009 from DH5α
07/08/13
- Digestion of Bba_K592009 by NotI, XhoI, EcoRI, EcoRI/PstI
- Electrophoresis of the digestion of Bba_K592009 by NotI, XhoI, EcoRI, EcoRI/PstI
07/12/13
- Transformation of Bba_B0015, Bba_B0017
- Design of RBS-AmilCP oligos
07/15/13
- Liquid culture of Bba_B0015, Bba_B0017
07/16/13
- Extraction of Bba_B0015, Bba_B0017 from DH5α
- Digestion of Bba_B0015, Bba_B0017, Bba_I732019 by EcoRI/PstI
07/18/13
- PCR of Bba_B0015, Bba_B0017 digested by EcoRI/PstI
07/19/13
- Electrophoresis of Colony PCR products : Bba_B0015, BBa_B0017
- Gel purification of the electrophoresis of Colony PCR products : Bba_B0015, BBa_B0017
07/22/13
- Digestion of Bba_K592009 by SpeI/PstI, Bba_B0015 and Bba_B0017 by PstI/XbaI
- Denaturation of SpeI/PstI and PstI/XbaI used for the digestion of Bba_K592009, Bba_B0015 and Bba_B0017
07/23/13
- Electrophoresis of the digestion of Bba_K592009 by SpeI/PstI
- Electrophoresis of the digestion of Bba_B0015, Bba_B0017 by XbaI/PstI
- Digestion of Bba_K592009 by SpeI/PstI
- Electrophoresis to check the digestion of Bba_K592009 by SpeI/PstI
- Liquid culture of Bba_K592009 in DH5α
07/24/13
- Extraction of Bba_K592009 from DH5α
07/25/13
- Gel purification of Term
- Electrophoresis to check the gel purification of Term
- PCR of Bba_K592209 with RBS-Amil CP primer and suffix : PSB1C3
- Digestion of Colony PCR products : RBS-Amil CP by EcoRI/SpeI
07/26/13
- Denaturation of EcoRI/SpeI used for the digestion of PCR products : RBS-AmilCP
- Ligation of RBS-AmilCP and Term-PSB1C3
07/30/13
- Tranformation of Bba_K1155003 in DH5α
Bba_K1155004
07/25/13
- Colony PCR of NirB
- Electrophoresis to check the colony PCR of NirB
- Colony PCR of NirB
07/26/13
- Electrophoresis of PCR products : NirB [File:Pswrong.png|20px]]
- PCR of NirB
- Electrophoresis of PCR products : NirB
- Gel purification of PCR products : NarK
- Digestion of PCR products : NarK by EcoRI/PstI
07/30/13
- Denaturation of EcoRI/SpeI used of the digestion of PCR products : NirB
07/31/13
- Ligation of NirB in PSB1C3
- Electrophoresis to check the ligation of NirB in PSB1C3
- Transformation of Bba_K1155004
Bba_K1155005
07/25/13
- Colony PCR of nag
- Electrophoresis to check the colony PCR of NarG
- Colony PCR of NarG
07/26/13
- Electrophoresis of PCR products : NarG [File:Pswrong.png|20px]]
- PCR of NarG
- Electrophoresis of PCR products : NarG
- Gel purification of PCR products : NarG
- Digestion of PCR products : NarG by EcoRI/PstI
07/30/13
- Denaturation of EcoRI/SpeI used of the digestion of PCR products : NarG
07/31/13
- Ligation of NarG in PSB1C3
- Electrophoresis to check the ligation of NarG in PSB1C3
- Transformation of Bba_K1155005
Bba_K1155006
07/25/13
- Colony PCR of NarK
- Electrophoresis to check the colony PCR of NarK
- Colony PCR of NarK
07/26/13
- Electrophoresis of PCR products : NarK [File:Pswrong.png|20px]]
- PCR of NarK
- Electrophoresis of PCR products : NarK
- Gel purification of PCR products : NirB
- Digestion of PCR products : NirB by EcoRI/PstI
07/30/13
- Denaturation of EcoRI/SpeI used of the digestion of PCR products : NarK
07/31/13
- Ligation of NarK in PSB1C3
- Electrophoresis to check the ligation of NarK in PSB1C3
- Transformation of Bba_K1155006
Bba_K1155007
07/02/13
- Tranformation of Bba_I732017 in DH5α
07/03/13
- New culture of Bba_I732017
07/05/13
- Extraction of Bba_I732017 from DH5α
07/08/13
- Digestion of Bba_I732017 by NotI, XhoI, EcoRI, EcoRI/PstI
- Electrophoresis of the digestion of Bba_I732017 by NotI, XhoI, EcoRI, EcoRI/PstI
07/10/13
- Transformation of Bba_I732019 in DH5α
07/12/13
- Transformation of Bba_I732019, Bba_B0015, Bba_B0017
07/15/13
- Liquid culture of Bba_B0015, Bba_B0017, Bba_I732019
07/16/13
- Extraction of Bba_B0015, Bba_B0017, Bba_I732019 from DH5α
- Digestion of Bba_B0015, Bba_B0017, Bba_I732019 by EcoRI/PstI
07/17/13
- Electrophoresis of digestions of Bba_B0015, Bba_B0017 by EcoRI/PstI
- Electrophoresis of digestions of Bba_I732019 by EcoRI/PstI
07/18/13
- PCR of Bba_B0015, Bba_B0017 digested by EcoRI/PstI
07/19/13
- Electrophoresis of Colony PCR products : Bba_B0015, BBa_B0017
- Gel purification of the electrophoresis of Colony PCR products : Bba_B0015, BBa_B0017
07/22/13
- Digestion of Bba_I732017 by EcoRI/SpeI, Bba_B0015 and Bba_B0017 by PstI/XbaI
- Denaturation of EcoRI/SpeI and PstI/XbaI used for the digestion of Bba_I732017, Bba_K502009, Bba_B0015 and Bba_B0017
07/23/13
- Electrophoresis of the digestion of Bba_I732017 by EcoRI/SpeI, Bba_B0015, Bba_B0017 by XbaI/PstI
- Gel purification of digestion products : RBS-LacZ
- Electrophoresis to check the gel purification of digestion products : RBS-LacZ
- Digestion of Bba_I732017 by EcoRI/Pst
- Electrophoresis to check the gel digestion of Bba_I732017 by EcoRI/SpeI
- Liquid culture of Bba_I732017 in DH5α
07/24/13
- Extraction of Bba_I732017 from DH5α
07/25/13
- Gel purification of Term
- Electrophoresis to check the gel purification of Term
07/30/13
- Digestion of Bba_I732017 by EcoRI/PstI
- Electrophoresis to check the digestion of Bba_I732017 by EcoRI/PstI [File:Psright.png|20px]]
- Gel purification of digestion of Bba_I732017 by EcoRI/PstI
- Digestion of Bba_I732017 by EcoRI/PstI
- Electrophoresis to check the digestion of Bba_I732017 by EcoRI/SpeI [File:Psright.png|20px]]
- Gel purification of digestion of Bba_I732017 by EcoRI/PstI
07/31/13
- Digestion of Bba_I732017 by EcoRI/SpeI
- Electrophoresis to check the digestion of Bba_I732017 by EcoRI/SpeI [File:Psright.png|20px]]
- Gel purification of digestion of RBS-LacZ
Bba_K1155008
07/30/13
- PCR of : FNR Part I, FNR Part II, RBS-FNR Part I
- Electrophoresis of the PCR products : FNR Part I, FNR Part II, RBS-FNR Part I
Bba_K1155009
07/01/13
- Design of oligos for amplification of BphR2 gene of Pseudomonas pseudoalcaligenes, Pseudomonas oleovorans
07/09/13
- PCR purification of BphR2
- Digestion PCR products : BphR2 and digestion of PSB1C3
- Denaturation of EcoRI/PstI used for the digestion of PCR products : BphR2 and PSB1C3
- Ligation of BphR2 and PSB1C3
07/10/13
- Transformation of ligation of PSB1C3 and BphR2
07/11/13
- Colony PCR of BphR2 in PSB1C3 in DH5α to check good insertion of BphR2 in PSB1C3
- Electrophoresis of Colony PCR products : BphR2 in PSB1C3
07/12/13
- Stock of clones 3, 4 for BphR2
- Streak of BphR2
- Extraction of clones 3, 4 for BphR2 from DH5α
07/15/13
- Digestion of BphR2 in PSB1C3 by EcoRI/PstI and SacII to check the size of fragments
- PCR of digestion of BphR2 in PSB1C3
07/16/13
- Electrophoresis of the digestion of BphR2 in PSB1C3
07/30/13
- PCR of : BphR2 Part I, BphR2 Part II, RBS-BphR2 Part I
- Electrophoresis of the PCR products : BphR2 Part I, BphR2 Part II
- Electrophoresis of the PCR products : RBS-BphR2 Part I
- PCR of : RBS-BphR2 Part I
- Gel purification of PCR of : RBS-BphR2 Part I
PSB3K3
07/09/13
- Transformation of Bba_J04450 in DH5α
07/10/13
- Mini and maxi preparation of the culture of Bba_J04450
07/11/13
- Extraction of Bba_J04450 from DH5α
- Digestion of Bba_J04450 to chek the size for the plasmid
- Electrophoresis of the digestion of Bba_J04450
07/16/13
- Digestion of PSB3K3 by EcoRI/PstI
- Electrophoresis of the digestion of PSB3K3 by EcoRI/PstI
- Gel purification of electrophoresis of the digestion of PSB3K3 by EcoRI/PstI
