Team:British Columbia/Notebook/CRISPR

From 2013.igem.org

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=Team CRISPR=
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===July 3===
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*PAM + spacer (on Amp)
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==='''July 10th'''===
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'''Results:''' Unsuccessful no bands were seen on the agarose gel.
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Revision as of 05:39, 27 September 2013

iGEM Home

Contents

Team CRISPR

July 3

List of BioBricks that are/will be on the way:

Cas9:

  • Cas9 - Joe and Anna
  • pTet -
  • const. promoter + Cas9 - Joe
  • promoter + Cas9 + Leader + R + spacer + R (on Kan)
  • L + R + spacer + R

Target:

  • tracerRNA
  • repeat + spacer + repeat - Vanins*2 + underlings
  • leader - Ray, Fisal, Dan

Decoy:

  • PAM + spacer (on Amp)

July 10th

Experimenter: Anna Müller

Aim: PCR the cas9 gene from Streptococcus thermophilus cells.

Results: Unsuccessful no bands were seen on the agarose gel.

September 22nd

Experimenter: Anna Müller

Aim: Digest T4, T7 spacer sequence with PAM site(decoy construct) with EcoRI and SpeI to later on ligate into plasmid with ampicillin resistance.

Results: will follow.


Aim: Digest PsBIA3 plasmid with ampicillin resistance with EcoRI and SpeI to construct the decoy plasmides.

Results: will follow.


Retrieved from "http://2013.igem.org/Team:British_Columbia/Notebook/CRISPR"