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Team:ZJU-China/Project/GhostSensor/Perspectives
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Our ghost sensor platform can not only be used to detect proteins, it can also be used to specifically clear proteins in solution and achieve long-term storage of proteins. | Our ghost sensor platform can not only be used to detect proteins, it can also be used to specifically clear proteins in solution and achieve long-term storage of proteins. | ||
| - | === Ghost | + | === Ghost cleaner === |
| - | We can deliberately deplete a specific kind of protein from solution with an antibody pull down assay, but it is too expensive to perform and need a great amount of antibodies. Using ghost sensor platform is good alternative. All we have to do is adding bacterial ghost into the solution in which streptavidin is fused to inner-membrane scaffold. Then aptamers specific to the target are included to the solution. Bacterial ghosts act like a giant magnet, suck large amounts of targeted molecules into their bodies. At last, we can centrifuge to precipitate bacterial ghosts together with targeted molecules. Because the size of holes in the bacteria surface can be adjusted by controlling the concentration of magnesium during induction, our ghost sensor platform can in principle clear a variety of targets ranging from small chemicals to viruses. | + | We can deliberately deplete a specific kind of protein from solution with an antibody pull down assay, but it is too expensive to perform and need a great amount of antibodies. Using ghost sensor platform is good alternative. All we have to do is adding bacterial ghost into the solution in which streptavidin is fused to inner-membrane scaffold. Then aptamers specific to the target are included to the solution. Bacterial ghosts act like a giant magnet, suck large amounts of targeted molecules into their bodies. At last, we can centrifuge to precipitate bacterial ghosts together with targeted molecules. Because the size of holes in the bacteria surface can be adjusted by controlling the concentration of magnesium during induction, our ghost sensor platform can in principle clear a variety of targets ranging from small chemicals to viruses. |
=== Ghost store === | === Ghost store === | ||
Revision as of 06:22, 27 September 2013
Ghost Sensor: Perspectives
Our ghost sensor platform can not only be used to detect proteins, it can also be used to specifically clear proteins in solution and achieve long-term storage of proteins.
Ghost cleaner
We can deliberately deplete a specific kind of protein from solution with an antibody pull down assay, but it is too expensive to perform and need a great amount of antibodies. Using ghost sensor platform is good alternative. All we have to do is adding bacterial ghost into the solution in which streptavidin is fused to inner-membrane scaffold. Then aptamers specific to the target are included to the solution. Bacterial ghosts act like a giant magnet, suck large amounts of targeted molecules into their bodies. At last, we can centrifuge to precipitate bacterial ghosts together with targeted molecules. Because the size of holes in the bacteria surface can be adjusted by controlling the concentration of magnesium during induction, our ghost sensor platform can in principle clear a variety of targets ranging from small chemicals to viruses.
Ghost store
In our team’s safety section, we tried to construct CaCO3 bacterial shell to elongate E.coli storage time. This technique can also be applied to ghost sensor platform. When targeted proteins are precipitated from the solution with bacterial ghost, we build a shell around our E.coli to completely seal the tunnels. In this way, proteins are insulated from harsh outside environment and kept relatively safe bathing the buffer system inside the shell. We hope that this technique could tackle the problem that proteins cannot be long-term stored in room temperature due to proteinase in the environment.
