Team:Hong Kong HKUST/results

From 2013.igem.org

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<p id="yo"><font size=3>Our project was first proposed in mid-March and after a series of improvement and elaboration, we started our wet lab work from mid-June to late-September. After 3 months of memorable diligent work, we are proud to claim that we have achieved the results below:
<p id="yo"><font size=3>Our project was first proposed in mid-March and after a series of improvement and elaboration, we started our wet lab work from mid-June to late-September. After 3 months of memorable diligent work, we are proud to claim that we have achieved the results below:
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<ul>
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   <li>Successfully constructed the two parts</a> for mitochondria leader sequence, MLS with RFC 10 and RFC 25, (<a href="http://parts.igem.org/Part:BBa_K1119000">BBa_K1119000</a>, <a href="http://parts.igem.org/Part:BBa_K1119001">BBa_K1119001</a>).</li>
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   <li>Successfully constructed two parts</a> carrying mitochondrial leader sequence (MLS) compatible with RFC 10 and RFC 25 assembly standards respectively, (<a href="http://parts.igem.org/Part:BBa_K1119000">BBa_K1119000</a>, <a href="http://parts.igem.org/Part:BBa_K1119001">BBa_K1119001</a>).</li>
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   <li>Successfully constructed the </a>part of  aceA and aceB for in glyoxylate shunt (<a href="http://parts.igem.org/Part:BBa_K1119002">BBa_K1119002</a>, <a href="http://parts.igem.org/Part:BBa_K1119003">BBa_K1119003</a>). </li>
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   <li>Successfully constructed </a>parts containing the<i>aceA</i> and <i>aceB</i> elements of the glyoxylate shunt (<a href="http://parts.igem.org/Part:BBa_K1119002">BBa_K1119002</a>, <a href="http://parts.igem.org/Part:BBa_K1119003">BBa_K1119003</a>). </li>
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   <li>Successfully constructed the part of a constitutive regulatory promoter, Human Elongation Factor 1-alpha promoter (<a href="http://parts.igem.org/Part:BBa_K1119004">BBa_K1119004</a>).</li>
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   <li>Successfully constructed a part containing the constitutive regulatory promoter of Human Elongation Factor 1-alpha (<a href="http://parts.igem.org/Part:BBa_K1119004">BBa_K1119004</a>).</li>
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   <li>Successfully construct the part for a constitutive CMV promoter (<a href="http://parts.igem.org/Part:BBa_K1119006">BBa_K1119006</a>).</li>
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   <li>Successfully constructed a part containing the constitutive P<i>cmv</i> promoter (<a href="http://parts.igem.org/Part:BBa_K1119006">BBa_K1119006</a>).</li>
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   <li>Successfully characterized the regulatory promoter <em>pCMV</em> in HEK293FT cells (<a href="http://parts.igem.org/Part:BBa_K1119008">BBa_K1119008</a>).</li>
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   <li>Successfully characterized the constitutive promoter P<i>cmv</i> in HEK293FT cells (<a href="http://parts.igem.org/Part:BBa_K1119008">BBa_K1119008</a>).</li>
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   <li>Successfully characterized the mitochondria leader sequence MLS in HEK293FT cells (<a href="http://parts.igem.org/Part:BBa_K1119009">BBa_K1119009</a>).</li>
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   <li>Successfully characterized the mitochondrial leader sequence (MLS) in HEK293FT cells (<a href="http://parts.igem.org/Part:BBa_K1119009">BBa_K1119009</a>).</li>
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   <li>Successfully tested the HepG2 cell viability in different sodium palmitate concentrations. </li>
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   <li>Successfully tested HepG2 cell viability in different sodium palmitate concentrations. </li>
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</ul></font></p>
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</ul></p>
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Revision as of 16:12, 27 September 2013





Result


Our project was first proposed in mid-March and after a series of improvement and elaboration, we started our wet lab work from mid-June to late-September. After 3 months of memorable diligent work, we are proud to claim that we have achieved the results below:

  • Successfully constructed two parts carrying mitochondrial leader sequence (MLS) compatible with RFC 10 and RFC 25 assembly standards respectively, (BBa_K1119000, BBa_K1119001).
  • Successfully constructed parts containing theaceA and aceB elements of the glyoxylate shunt (BBa_K1119002, BBa_K1119003).
  • Successfully constructed a part containing the constitutive regulatory promoter of Human Elongation Factor 1-alpha (BBa_K1119004).
  • Successfully constructed a part containing the constitutive Pcmv promoter (BBa_K1119006).
  • Successfully characterized the constitutive promoter Pcmv in HEK293FT cells (BBa_K1119008).
  • Successfully characterized the mitochondrial leader sequence (MLS) in HEK293FT cells (BBa_K1119009).
  • Successfully tested HepG2 cell viability in different sodium palmitate concentrations.



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