Team:Hong Kong HKUST/modules

From 2013.igem.org

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Revision as of 16:13, 27 September 2013

Project Outline

Our ultimate goal is to build a ‘smart’ glyoxylate shunt that burns extra fatty acid there is a surplus of energy. To achieve this, we would have to first build the shunt itself in mammalian cells, which consists of two bacterial enzymes, isocitrate lyase (AceA) and malate synthase (AceB). These two glyoxylate enzymes were tagged with localization signal peptide, so they could be directed to the mitochondria and act on the citric acid cycle, eventually increasing fatty acid uptake. The two enzymes were initially driven by constitutive promoters (CMV and EF-1alpha promoters), which put the shunt in a constantly “ON” state and burns calories regardless of the energy profile. To improve this, we are developing fatty acid responsive promoters, which, when used to regulate the glyoxylate enzymes, should dispense energy only when it is in excess. Hover your mouse and click on the images below to learn more about each modules!

Cell Viability & Fatty Acid Quantification

Responsible for:
Measuring cell viability at different fatty acid concentration & measure fatty acid uptake rate
Parts submitted: -

Fatty Acid Sensing Mechanism

Responsible for:
Introduce inducible system that allows tunable fatty acid uptake by sensing fatty acid concentration
Parts submitted: -

Protein Trafficking

Responsible for:
Target ACE proteins into mitochondria
Parts submitted: BBa_K1119000, BBa_K1119001 & BBa_K1119009

Glyoxylate Shunt

Responsible for:
Introduce glyoxylate enzymes responsible for the shunt
Parts submitted:BBa_K1119002, BBa_K1119003, BBa_K1119004, BBa_K1119006 & BBa_K1119008

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-<br><br><br><div id="slide"><h3 class="title">Project Outline</h3><p id="isi">Our ultimate goal is to build a ‘smart’ glyoxylate shunt that increases energy metabolism by accelerating fatty acid uptake rate when there is a surplus of energy. To achieve this, we would have to first build the shunt itself in mammalian cells, which consists of two bacterial enzymes, isocitrate lyase (AceA) and malate synthase (AceB). These two glyoxylate enzymes were tagged with mitochondrial leader sequence, so they could be directed to the compartment where the citric acid cycle could be effected on. The two enzymes were initially driven by constitutive promoters (CMV and EF-1alpha promoters), which put the shunt in a constantly “ON” state and burns calories regardless of the energy profile. To improve this, we are developing fatty acid responsive promoters, which, when used to regulate the glyoxylate enzymes, should dispense energy only when it is in excess. <strong>Hover</strong> your mouse and <strong>click</strong> on the images below to learn more about each modules!</p></div>
+<br><br><br><div id="slide"><h3 class="title">Project Outline</h3><p id="isi">Our ultimate goal is to build a ‘smart’ glyoxylate shunt that burns extra fatty acid there is a surplus of energy. To achieve this, we would have to first build the shunt itself in mammalian cells, which consists of two bacterial enzymes, isocitrate lyase (AceA) and malate synthase (AceB). These two glyoxylate enzymes were tagged with localization signal peptide, so they could be directed to the mitochondria and act on the citric acid cycle, eventually increasing fatty acid uptake. The two enzymes were initially driven by constitutive promoters (CMV and EF-1alpha promoters), which put the shunt in a constantly “ON” state and burns calories regardless of the energy profile. To improve this, we are developing fatty acid responsive promoters, which, when used to regulate the glyoxylate enzymes, should dispense energy only when it is in excess. <strong>Hover</strong> your mouse and <strong>click</strong> on the images below to learn more about each modules!</p></div>
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