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Team:Hong Kong HKUST/Project/module3
From 2013.igem.org
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| - | In vertebrates, the signal sequence coding region promotes translocation of precursor peptide. In our project, we introduced bacterial enzymes to mammalian cell to modify metabolic pathway. However, unlike bacteria, citric acid cycle in mammalian cells is compartmentalized in mitochondria. The glyoxylate enzymes (AceA and AceB proteins, denoted ACE enzymes) should be targeted to mitochondria for their functionality. To do so, we fused ACE enzymes with Mitochondrial Leader Sequence (MLS). We have constructed and qualitatively and quantitatively <a href="https://2013.igem.org/Team:Hong_Kong_HKUST/characterization/mls">characterized</a> Mitochondrial Leader Sequence BioBrick <a href="http://parts.igem.org/Part:BBa_K1119001">BBa_K1119001</a>. | + | In vertebrates, the signal sequence coding region promotes translocation of precursor peptide. In our project, we introduced bacterial enzymes to mammalian cell to modify metabolic pathway. However, unlike bacteria, citric acid cycle in mammalian cells is compartmentalized in mitochondria. The glyoxylate enzymes (<a href="http://parts.igem.org/Part:BBa_K1119002">AceA</a> and <a href="http://parts.igem.org/Part:BBa_K1119003">AceB</a> proteins, denoted ACE enzymes) should be targeted to mitochondria for their functionality. To do so, we fused ACE enzymes with Mitochondrial Leader Sequence (MLS). We have constructed and qualitatively and quantitatively <a href="https://2013.igem.org/Team:Hong_Kong_HKUST/characterization/mls">characterized</a> Mitochondrial Leader Sequence BioBrick <a href="http://parts.igem.org/Part:BBa_K1119001">BBa_K1119001</a>. |
</div> | </div> | ||
Revision as of 16:48, 27 September 2013
-
Protein Trafficking
- Overview
- Mechanism of MLS
- Linkage to Project
- Reference
-
Modules
- FA Quantification & Cell Viability
- FA Sensing Mechanism
- Protein Trafficking
- Glyoxylate Shunt
Protein Trafficking
Overview
In vertebrates, the signal sequence coding region promotes translocation of precursor peptide. In our project, we introduced bacterial enzymes to mammalian cell to modify metabolic pathway. However, unlike bacteria, citric acid cycle in mammalian cells is compartmentalized in mitochondria. The glyoxylate enzymes (AceA and AceB proteins, denoted ACE enzymes) should be targeted to mitochondria for their functionality. To do so, we fused ACE enzymes with Mitochondrial Leader Sequence (MLS). We have constructed and qualitatively and quantitatively characterized Mitochondrial Leader Sequence BioBrick BBa_K1119001.Mechanism of MLS
MLS is attached to the N-terminal of enzyme, and bind to the receptor protein on mitochondrial membrane, and diffuse to contact site where inner and outer membrane fuse, then bring the ACE enzyme into mitochondria. Afterward, it is be cleaved, leaving the enzyme in mitochondria.
