Team:UFMG Brazil/lab
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* Repeat step 6, but using 1 mL of cold solution of CaCl2 to resuspend the cells. (Note: In this solution, cells can stay from 12 to 24 hours) | * Repeat step 6, but using 1 mL of cold solution of CaCl2 to resuspend the cells. (Note: In this solution, cells can stay from 12 to 24 hours) | ||
* Divide the cells in aliquots of 100 μL and freeze it at -80°C. | * Divide the cells in aliquots of 100 μL and freeze it at -80°C. | ||
+ | |||
+ | CaCl2 solution (100 mL): | ||
+ | |||
+ | * 60 mM <html>CaCl<sub>2</sub></html> - 0.882 g | ||
+ | * Glycerol 15% - 15 mL | ||
+ | * 10 mM PIPES - 0.3785 g (Note: Do not use PIPES free acid | ||
+ | * Sterilize (autoclave) and store at room temperature | ||
Revision as of 19:05, 27 September 2013