Team:UFMG Brazil/lab
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* Add 10 mL H2O to cobalt. Homogenize the mixture. | * Add 10 mL H2O to cobalt. Homogenize the mixture. | ||
* Filter it using a 0.22 <html>μ</html>m strainer. | * Filter it using a 0.22 <html>μ</html>m strainer. | ||
+ | |||
+ | '''4. DNA digestion''' | ||
+ | |||
+ | A) Single Reaction <html>μ</html>M | ||
+ | * DNA - 125 ng | ||
+ | * Buffer 10X* - 1 <html>μ</html>L | ||
+ | * BSA 10 <html>μ</html>g/mL - 1 <html>μ</html>L | ||
+ | * Enzyme* - 0.25 <html>μ</html>L | ||
+ | * ddH2O (di-deionized) - complet to 10 <html>μ</html>L | ||
Revision as of 19:23, 27 September 2013