Team:Hong Kong HKUST/experiment/exp4
From 2013.igem.org
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- | < | + | <h1>Overview</h1> |
- | <p id="yo"> | + | <p id="yo">Our artificial futile cycle is driven by the expression of two key enzymes in glyoxylate cycle. Since mammalian cells lack genes expressing glyoxylate shunt, we introduce these genes form <i>E. coli</i> and assemble them in a constitutive construct that will allow the expression of prokaryotic gene in eukaryotic cell. In addition to the constitutive system, we will also assemble an inducible construct which will allow tunable gene expression according to the concentration of fatty acid in the medium. We decided to introduce inducible system to prevent fatty acid deficiency in low concentration of plasma fatty acid and facilitate greater fatty acid uptake at a high circulating fatty acid levels. The inducible and constitutive system will be compared in terms of fatty acid uptake rate in a range concentration of fatty acid. </p> |
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<h3>A Sneak Peek of Our Blog</h3><br> | <h3>A Sneak Peek of Our Blog</h3><br> | ||
<center><img src="https://static.igem.org/mediawiki/2013/2/2e/Blog-cecilia.png" style="width:90%"></center><br> | <center><img src="https://static.igem.org/mediawiki/2013/2/2e/Blog-cecilia.png" style="width:90%"></center><br> |
Revision as of 20:37, 27 September 2013
Glyoxylate Shunt
Overview
Our artificial futile cycle is driven by the expression of two key enzymes in glyoxylate cycle. Since mammalian cells lack genes expressing glyoxylate shunt, we introduce these genes form E. coli and assemble them in a constitutive construct that will allow the expression of prokaryotic gene in eukaryotic cell. In addition to the constitutive system, we will also assemble an inducible construct which will allow tunable gene expression according to the concentration of fatty acid in the medium. We decided to introduce inducible system to prevent fatty acid deficiency in low concentration of plasma fatty acid and facilitate greater fatty acid uptake at a high circulating fatty acid levels. The inducible and constitutive system will be compared in terms of fatty acid uptake rate in a range concentration of fatty acid.
A Sneak Peek of Our Blog
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