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Team:BYU Provo/Notebook/Cholera - Enzymes/Protocols
From 2013.igem.org
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*0.5 ul Phusion Polymerase | *0.5 ul Phusion Polymerase | ||
| - | Set the PCR machine to run the following cycle with the middle three being repeated 35 times | + | Set the PCR machine to run the following cycle with the middle three phases being repeated 35 times |
*98°C for 02:00 | *98°C for 02:00 | ||
*98°C for 00:30 | *98°C for 00:30 | ||
| Line 92: | Line 92: | ||
*0.5 ul ''TAQ'' Polymerase | *0.5 ul ''TAQ'' Polymerase | ||
| + | Set the PCR machine to run the following cycle with the middle three phases being repeated 35 times | ||
*95 for 2:00 | *95 for 2:00 | ||
*95 for 0:30 | *95 for 0:30 | ||
Revision as of 21:47, 27 September 2013
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Cholera-Enzyme Protocols
Salt LB recipe
To 950 ml distilled H2O add
Adjust pH to 7.8
Fill to 1000 ml and autoclave
Phusion PCR (50ul reaction)
For each sample add:
Set the PCR machine to run the following cycle with the middle three phases being repeated 35 times
Hold at 4°C
Taq polymerase PCR (50ul reaction)
For each sample add:
Set the PCR machine to run the following cycle with the middle three phases being repeated 35 times
===Any deviations from these protocols are listed in the notebook when they were used=== |
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