Team:BYU Provo/Notebook/Cholera - Enzymes/May-June

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Revision as of 23:40, 27 September 2013

Cholera - Enzyme Notebook May - June
Cholera Enzyme March-April May-June July-August September-October Protocols	May 1 - May 14 We started contacting teams that have worked with Savinase and DspB. We started the initial work of purifying AmyA which was available at BYU. As well we started outlining the biofilm degradation assay using positive and negative controls. Daily log
	May 15 - May 31 Continued our work of cloning AmyA and Savinase into E. coli. Ran initial biofilm degradation assays using urea as a positive control. Daily log
	June 1 - June 14 We are still having issues with AmyA and Savinase. We are starting to fine tune our biofilm degradation assay. Daily log
	June 15 - June 30 After a few more failed attempts with Savinase we are restarting the isolation from B. subtilis. AmyA is almost finished and ready for purification. The biofilm degradation assay is still being fine tuned. Daily log

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