Revision as of 00:44, 28 September 2013

Parts

The following parts were assembled throughout the development of this project:

Characterization	Name	Type	Description	Designer
BBa_K1212002	Plasmid_Backbone	Golden Gate Compatible pSB3K3	Alex Neckelmann	2750
BBa_K1212006	Composite	pBAD+Golden Gate Compatible GFP	Aura Ferreiro	1052
BBa_K1212009	Composite	pBAD+Riboswitch 2+GFP+T	Amy Soon	1086
BBa_K1212010	Composite	pBAD+Riboswitch 1+GFP+T	Amy Soon	1090
BBa_K1212011	Composite	pBAD+Riboswitch 2+TAL1	Amy Soon	2612
BBa_K1212012	Composite	pBAD+Riboswitch 2+TAL8	Amy Soon	2616
BBa_K1212013	Composite	pTet+TBS 1+RBS+GFP+T	Aura Ferreiro	1003
BBa_K1212014	Composite	pBAD+Riboswitch 1+TAL 1	Amy Soon	2616
BBa_K1212015	Composite	pBAD+Riboswitch 1+TAL 8	Amy Soon	2620
BBa_K1212016	Composite	pTet+TBS 2+RBS+GFP	Amy Soon	1003

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-Our best characterized parts are our RiboTAL constructs, which consist of parts BBa_K1212011-BBa_K1212016. Among these parts, most of characterization was focused on the riboswitch+TAL constructs (BBa_K1212011, BBa_K1212012, BBa_K1212014, BBa_K1212015), as the other parts were simply pTet+GFP, which are already well characterized parts, with some extra base pairs added for our TAL binding sites (BBa_K1212013, BBa_K1212016). Both our pSB3K3 backbone (BBa_K1212002) and our GFP (K1212006) with their BsaI sites fixed were used in our RiboTAL constructs and functioned as expected but did not need separate characterization.
+Our best characterized parts are our RiboTAL constructs, which consist of parts BBa_K1212011-BBa_K1212016. Among these parts, most of characterization was focused on the riboswitch+TAL constructs (BBa_K1212011, BBa_K1212012, BBa_K1212014, BBa_K1212015), as the other parts were simply pTet+GFP, which are already well characterized parts, with some extra base pairs added for our TAL binding sites (BBa_K1212013, BBa_K1212016). Both our pSB3K3 backbone (BBa_K1212002) and our GFP (BBa_K1212006) with their BsaI sites fixed were used in our RiboTAL constructs and functioned as expected but did not need separate characterization.
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Project Background Learn about how we combine riboswitches and TALs into robust orthogonal mechanisms for inducible repression.	Results Check out the results of our experiments.
Human Practices Take a look at how we designed a new database for better raw data characterization of Biobricks.	Judging Criteria Here's the criteria that we met for this year's team.