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Team:Paris Saclay/Notebook/August/20
From 2013.igem.org
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Damir, XiaoJing | Damir, XiaoJing | ||
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| + | Sequencies were good. We obtain : Bba_K1155004, Bba_K1155005, Bba_K1155006. | ||
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==='''A - Aerobic/Anaerobic regulation system / B - PCB sensor system'''=== | ==='''A - Aerobic/Anaerobic regulation system / B - PCB sensor system'''=== | ||
Revision as of 14:44, 28 September 2013
Contents |
Notebook : August 20
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Bba_K1155004, Bba_K1155005, Bba_K1155006
1 - Sequences analysis
Damir, XiaoJing
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Sequencies were good. We obtain : Bba_K1155004, Bba_K1155005, Bba_K1155006. |
A - Aerobic/Anaerobic regulation system / B - PCB sensor system
Objective : obtaining FNR and BphR2 proteins
1 - PCR of BphR2 Part I
Damir
Used quantities :
- Oligo 54F : 2µL
- Oligo 55R : 2µL
- DNA : 1µL
- Buffer Phusion : 10µL
- dNTP : 1µL
- Phusion : 1µL
- DMS9 : 2µL ??????????????????????????????????
- H2O : 31µL
PCR program :
2 - Electrophoresis of PCR products : BphR2 Part I
Nadia
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We obtain a frangment at the right size. We can purify it. |
2 - Gel purification of PCR products : BphR2 Part I
Damir, Nadia
Protocol : [http://www.mn-net.com/tabid/1452/default.aspx Gel purification ]
Nanodrop :
- BphR2 Part I: .........
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We lost our fragment. We will do the PCR again. |
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