Team:Uppsala/collaboration

From 2013.igem.org

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<h1>High school outreach</h1>
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        <p>Another important work in human practice was to spread the word about synthetic biology to the  
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      <h1 class="main-title">Astaxanthin</h1>
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younger generation. One if the first things we did in spring was to travel around to different high
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<p>Astaxanthin is a carotenoid in the carotenoid pathway that derives from the xanthophyll group and the precursor canthaxanthin is catalyzed by CrtZ. Astaxanthin is a very strong antioxidant but studies have shown that it also has other health benefits. The substance is created by many marine bacteria and microalgae and it gives the characteristic red color to many species such as salmon, lobster and shrimps.
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schools to spread the word about synthetic biology and iGEM. We have also started collaborations
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with biotechnology high school students to do projects together with them in the future, with the
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goal to spread the knowledge of synthetic biology.</p>
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<img class="high-school-pic" src="https://static.igem.org/mediawiki/2013/4/4d/Uppsala2013_High-school1.png.jpg">
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Astaxanthin is an expensive part of salmon farming, used to give the meat its red color and it is approved in many countries as a food coloring with the e-number 161.[1] When astaxanthin is  produced it is usually by using the microalgae Haematococcus, or by producing it synthetically. The costs for astaxanthin today is around 2500 US dollars per kg, in total the market is worth around 200 million US dollars a year.[2] Since astaxanthin has many health benefits and that there is a demand for it on the market we decided to do more work on it to make it more available.</p>
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<img class="high-school-pic2" src="https://static.igem.org/mediawiki/2013/c/cc/Uppsala2013_High-school2.png.jpg">
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        <img class="molecule" src="https://static.igem.org/mediawiki/2013/4/47/Uppsala2013_Asthaxanthin-molecule.png">
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<h1>Methods</h1>
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<p>To see that the bacteria containing the operon expressed astaxanthin we had to characterize them. To do that we used a spectrophotometer (nanodrop 2000 UV/Vis spectrophotometer) and we extracted astaxanthin from salmon as a standard. After growing bacteria cultures with the operon we later used different extraction methods to extract the astaxanthin with various results.
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<br><br>
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We got the operon for producing astaxanthin from the Slovenian iGEM team 2010 which consisted of the genes CrtE, CrtB, CrtI, CrtY, CrtO and CrtZ with RBS, linkers, zinc fingers and the inducible promoter pBAD/AraC. We inserted the operon in e-coli DH5alpha. The zinc fingers aren´t of much interest to us so we won´t use them for any other parts.</p>
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        <img class="methods-pic" src="https://static.igem.org/mediawiki/2013/4/46/Uppsala2013_Astaxanthin-plasmid.png">     
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<h1>Results</h1>
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<p>In the end we may have gotten a successful result but the extraction method was not good enough to get a clear graph proving the existence of astaxanthine, only an indication on its existence.</p>
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        <div id="image"><img class="falcon" src="https://static.igem.org/mediawiki/2013/a/a5/Uppsala2013_Astaxanthin.jpg">
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<i class="fig1"><b>Figure 1.</b> The extraction of astaxanthin using methanol and salmon to get a standard.</i>
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Revision as of 18:10, 28 September 2013

Astaxanthin

Astaxanthin is a carotenoid in the carotenoid pathway that derives from the xanthophyll group and the precursor canthaxanthin is catalyzed by CrtZ. Astaxanthin is a very strong antioxidant but studies have shown that it also has other health benefits. The substance is created by many marine bacteria and microalgae and it gives the characteristic red color to many species such as salmon, lobster and shrimps.

Astaxanthin is an expensive part of salmon farming, used to give the meat its red color and it is approved in many countries as a food coloring with the e-number 161.[1] When astaxanthin is produced it is usually by using the microalgae Haematococcus, or by producing it synthetically. The costs for astaxanthin today is around 2500 US dollars per kg, in total the market is worth around 200 million US dollars a year.[2] Since astaxanthin has many health benefits and that there is a demand for it on the market we decided to do more work on it to make it more available.

Methods

To see that the bacteria containing the operon expressed astaxanthin we had to characterize them. To do that we used a spectrophotometer (nanodrop 2000 UV/Vis spectrophotometer) and we extracted astaxanthin from salmon as a standard. After growing bacteria cultures with the operon we later used different extraction methods to extract the astaxanthin with various results.

We got the operon for producing astaxanthin from the Slovenian iGEM team 2010 which consisted of the genes CrtE, CrtB, CrtI, CrtY, CrtO and CrtZ with RBS, linkers, zinc fingers and the inducible promoter pBAD/AraC. We inserted the operon in e-coli DH5alpha. The zinc fingers aren´t of much interest to us so we won´t use them for any other parts.

Results

In the end we may have gotten a successful result but the extraction method was not good enough to get a clear graph proving the existence of astaxanthine, only an indication on its existence.

Figure 1. The extraction of astaxanthin using methanol and salmon to get a standard.

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