Team:Freiburg/Highlights

From 2013.igem.org

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We started by mutating the nickase sites in the Cas9 protein and generated a novel type of DNA binding protein that is relying on <b> protein-RNA-DNA </b> interaction.
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A mutated Cas9 derived protein without nickase function was our start. This is basically a DNA binding protein, that is relying on a <b>protein-RNA-DNA </b> interaction.
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By fusing <b>effector domains</b> to Cas9 we added new properties to the protein. The <b>activation domain VP16</b> is able to activate transcription of genes. The fusion of the <b>transcriptional repressor domain KRAB</b> leads to synthetic repressor of gene expression. Specific <b>chromatin modification</b> was achieved by fusing a histone methyl transferase G9a to dCas9. With this protein we are able to specifically repress endogenous gene expression. </p> <p> </p> <p>
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By fusing <b>effector domains</b> to Cas9 we altered the properties it in various ways.</p><p> The <b>activation domain VP16</b> is able to activate transcription of genes. The fusion of the <b>transcriptional repressor domain KRAB</b> leads to synthetic repressor of gene expression. Specific <b>chromatin modification</b> was achieved by fusing a histone methyl transferase G9a to dCas9. With this protein we are able to specifically repress endogenous gene expression. </p> <p> </p> <p>
   
   
We were able to induce our system on light stimulus. This was possible by using photorecetors of higher plants.
We were able to induce our system on light stimulus. This was possible by using photorecetors of higher plants.

Revision as of 12:57, 29 September 2013

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