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Team:Paris Saclay/Notebook/July/17
From 2013.igem.org
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==='''A - Aerobic/Anaerobic regulation system'''=== | ==='''A - Aerobic/Anaerobic regulation system'''=== | ||
| - | ===='''Objective : obtaining | + | ===='''Objective : obtaining BBa_K1155003, BBa_K1155007'''==== |
| - | ===='''1 - Electrophoresis of digestions of | + | ===='''1 - Electrophoresis of digestions of BBa_B0015, BBa_B0017, BBa_I732019 by EcoRI/PstI'''==== |
Anaïs, Sheng | Anaïs, Sheng | ||
| - | * | + | * BBa_B0015, BBa_B0017 : |
{| | {| | ||
| style="width:350px;border:1px solid black;" |[[File:Psgel11707.jpg|300px]] | | style="width:350px;border:1px solid black;" |[[File:Psgel11707.jpg|300px]] | ||
| style="width:350px;border:1px solid black;vertical-align:top;" | | | style="width:350px;border:1px solid black;vertical-align:top;" | | ||
| - | * Well 1 : 2µL | + | * Well 1 : 2µL BBa_B0015+1µL of 6X loading dye |
| - | * Well 2 : 2µL | + | * Well 2 : 2µL BBa_B0015+1µL of 6X loading dye |
| - | * Well 3 : 2µL | + | * Well 3 : 2µL BBa_B0015+1µL of 6X loading dye |
* Well 4 : 6µL DNA Ladder | * Well 4 : 6µL DNA Ladder | ||
| - | * Well 5 : 2µL | + | * Well 5 : 2µL BBa_B0017+1µL of 6X loading dye |
| - | * Well 6 : 2µL | + | * Well 6 : 2µL BBa_B0017+1µL of 6X loading dye |
| - | * Well 7 : 2µL | + | * Well 7 : 2µL BBa_B0017+1µL of 6X loading dye |
* Gel : 1% | * Gel : 1% | ||
|} | |} | ||
Expected sizes : | Expected sizes : | ||
| - | * | + | * BBa_B0015 digested by EcoRI/PstI : |
| - | * | + | * BBa_B0017 digested by EcoRI/PstI : |
| - | * | + | * BBa_I732019 : |
{| | {| | ||
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| style="width:350px;border:1px solid black;" |[[File:Psgel31707.jpg|300px]] | | style="width:350px;border:1px solid black;" |[[File:Psgel31707.jpg|300px]] | ||
| style="width:350px;border:1px solid black;vertical-align:top;" | | | style="width:350px;border:1px solid black;vertical-align:top;" | | ||
| - | * Well 1 : 5µL | + | * Well 1 : 5µL BBa_I732019 digested by EcoRI/PstI+1µL of 6X loading dye |
* Well 2 : 6µL DNA Ladder | * Well 2 : 6µL DNA Ladder | ||
* Gel : 1% | * Gel : 1% | ||
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{| | {| | ||
| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" | | | style="border:1px solid black;padding:5px;background-color:#DEDEDE;" | | ||
| - | We didn't obtain fragments at the right size. We will use | + | We didn't obtain fragments at the right size. We will use BBa_I732017. |
|} | |} | ||
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==='''B - PCB sensor system'''=== | ==='''B - PCB sensor system'''=== | ||
| - | ===='''Objective : obtaining | + | ===='''Objective : obtaining BBa_K1155001, BBa_K1155002 and BphR2'''==== |
| - | ===='''1 - Stock of | + | ===='''1 - Stock of BBa_K1155001'''==== |
Anaïs, Sheng | Anaïs, Sheng | ||
Used quantities : | Used quantities : | ||
| - | * | + | * BBa_K1155001 confirmed : 1 mL |
* Glycerol : 500µL glycerol. | * Glycerol : 500µL glycerol. | ||
We stocked them at -20°C. | We stocked them at -20°C. | ||
| - | ===='''2 - Extraction of | + | ===='''2 - Extraction of BBa_K1155001 from DH5α'''==== |
Anaïs, Sheng | Anaïs, Sheng | ||
Revision as of 09:49, 30 September 2013
Contents |
Notebook : July 17
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining BBa_K1155003, BBa_K1155007
1 - Electrophoresis of digestions of BBa_B0015, BBa_B0017, BBa_I732019 by EcoRI/PstI
Anaïs, Sheng
- BBa_B0015, BBa_B0017 :
|
|
Expected sizes :
- BBa_B0015 digested by EcoRI/PstI :
- BBa_B0017 digested by EcoRI/PstI :
- BBa_I732019 :
|
We obtain fragments at the right size. We will do a PCR of them. |
|
|
Expected sizes :
- RBS-LacZ-term : 3500bp
|
We didn't obtain fragments at the right size. We will use BBa_I732017. |
B - PCB sensor system
Objective : obtaining BBa_K1155001, BBa_K1155002 and BphR2
1 - Stock of BBa_K1155001
Anaïs, Sheng
Used quantities :
- BBa_K1155001 confirmed : 1 mL
- Glycerol : 500µL glycerol.
We stocked them at -20°C.
2 - Extraction of BBa_K1155001 from DH5α
Anaïs, Sheng
Protocol : Hight copy plamid extraction
Modeling
Meeting memo
Meeting Date: 17/07/2013
Meeting participants: Patrick Amar, Claire Toffano-Nioche, Abdou Mouhtare, Damir Damipator, Gabriel Gallin, Zhou Yi
Recorder: Zhou Yi
Hsim:
-Hsim is created for bio-chemical reaction simulation, it demonstrates bio-chemical systems which the scientists are already known.
-The molecules are simulated as spheres (in 2D or 3D). Initial positions and size of sphere are configurable.
-example of a Hsim command:
B + V -> R + V [probability]
-Molecules diffusion are simulated by movements of spheres, if the distance between two centers of the spheres is less than the sum of 2 radium, we consider that will lead to a collision.
-Computer takes a random sample and compare it to the probability which is specified in equation. If the value exceeds the threshold, we consider that the reaction takes place.
-Probability of success is associated with kinetics of reaction which depends on concentrations of molecules varying during the reaction.
-Time unit is 100ms. So movement of spheres is discreet.
-Spheres move randomly but could follow some stochastic rules (like Brownian movement).
Ethic about open source:
-Hsim is not an open source software, it belongs to the University of Paris Sud. However, it is a free software, we can download it free.
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