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Team:TU-Delft/FinalApplication
From 2013.igem.org
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<li>Dissolve the peptides. Signiferin was dissolved in water, in order to dissolve maximim 1M of NH4HCO3 was added in order to gain an end concentration of 5mM.</li> | <li>Dissolve the peptides. Signiferin was dissolved in water, in order to dissolve maximim 1M of NH4HCO3 was added in order to gain an end concentration of 5mM.</li> | ||
| - | <li>The peptide solutions were measured, using the nanodrop, and we determined to have an absorbance of 0.74 and 0.51 respectively.Using absorbance A= εcL=εc when L = 1cm [1] the concentration was measured to be </li> | + | <li>The peptide solutions were measured, using the nanodrop, and we determined to have an absorbance of 0.74 and 0.51 respectively.Using absorbance A= εcL=εc when L = 1cm [1] the concentration was measured to be 0.49mM for signiferin and 0.34mM for maximim</li> |
Revision as of 12:30, 30 September 2013
BandAid Application
Experiments
Aim: To check if it is feasible for the peptides to pass through the selected membranes (Cellulose Nitrate Membrane and PTFE Membrane).
Procedure:- Dissolve the peptides. Signiferin was dissolved in water, in order to dissolve maximim 1M of NH4HCO3 was added in order to gain an end concentration of 5mM.
- The peptide solutions were measured, using the nanodrop, and we determined to have an absorbance of 0.74 and 0.51 respectively.Using absorbance A= εcL=εc when L = 1cm [1] the concentration was measured to be 0.49mM for signiferin and 0.34mM for maximim
- In order to make diffusion measurable, we folded the membrane like a filter paper and placed it in the peptide solutiom
- The membrane is add
