Team:Freiburg/plasmids

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Revision as of 16:56, 30 September 2013


oligo list
oligo name Sequence 5'-3' description
oIG0000 TGAGCAAGAGGTAAGGG Sequencing Primer
oIG0001 AAACTGGTGGACAGCAC Sequencing Primer
oIG0001_new GGACAGCACCGACAAGG Sequencing Primer
oIG0002 AGCTGCCTGAGAAGTAC Sequencing Primer
oIG0003 TGGACCTGCTGTTCAAG Sequencing Primer
oIG0004 ACGAGCTCGTGAAAGTG Sequencing Primer
oIG0005 AGAATGACAAGCTGATCCG Sequencing Primer
oIG0006 TGGCCTATTCTGTGCTG Sequencing Primer
oIG0007 CAGGCCGAGAATATCATCC Sequencing Primer
oIG0008 CAAACAACAGATGGCTGGC Sequencing Primer
oIG0009 CTCCGATCAGGTTCTTC Sequencing Primer
oIG0010 GACAAGAAGTACAGCATCG Sequencing Primer
oIG0011 CCCGTAATGCAGAAGAAG Sequencing Primer
oIG0012 GAAGCGGAGCTACTAAC Sequencing Primer
oIG0013 GACAATGCGATGCAATTTC Sequencing Primer
oIG0014 CTTGGTCACCTTCAGCTTG Sequencing Primer
oIG0015 TTTCTTGGGTAGTTTGCAG Sequencing Primer
oIG0016 ACCAAAATCAACGGGAC Sequencing Primer
oIG0017 ACCAATGTGACCGAGTTCG Sequencing Primer
oIG0018 GCCAGCCATCTGTTGTTTG Sequencing Primer
oIG0019 AGAAGCGCGATCACATG Sequencing Primer
oIG0020 CTAATTACAGCCCGGAGGA Sequencing Primer
oIG0021 ACCAGAAAGTTAACTGG Sequencing Primer (Bb Michael fwd)
oIG0022 TGGAATTCGCGGCCGCTTCTAGATGGCCGGCGACAAGAAGTACAGCATCGG Start+RFC25 fv
oIG0023 CTGCAGCGGCCGCTACTAGTATTAACCGGTGTCGCCTCCCAGCTGAG Ende+RFC25 rev
oIG0024 TCCGGCCTTATCCAGTTCGC 2742 (C-->A) rv
oIG0025 GCGAACTGGATAAGGCCGGATTCATCAAGAGACAGCTGGTGG 2742(C-->A) fv
oIG0026 TTGCAGGTAGTACAGGTACAGCTTCTCGTTCTGAAGCTGGGTGTTTTCCACGG 2415(G-->T)/2448 (G-->A!) rev
oIG0027 TGTACCTGTACTACCTGCAAAATGGGCGGGATATGTACGTG 2415(G-->T)/2448(G-->A!) fv
oIG0028 TGGCTCCGCCGTCAATGTATCCGGCGTAGCCGTTCTTG 1080(C-->A) rev
oIG0029 ATACATTGACGGCGGAGCC 1080(C-->A) fv
oIG0031 GGGCGAACTGCAAAAGGGAAACGAACTGGCCC 3648(C-->T)/3660(G-->A!) fv
oIG0032 TTCCCTTTTGCAGTTCGCCCGCAGAGGCCAGCATTCTCTTC 3648(C-->G)/3660(G-->A!) rev
oIG0033 AGGCAGCCCCGCCATTAAGAAGGGCATCCTGCAAACAGTGAAGGTGGTGGACGAGCTCGTGAAAGTGATGGGACGGCACAAGCCCGAGAAC 2181 (C-->A)/2214(G-->A!)/2253(C-->A) fv
oIG0034 TCTTAATGGCGGGGCTGCCTGCCAGATTGGCAATGTGCTC 2181(C-->A)/2214(G-->A!)/2253(C-->A)rev
oIG0035 TGGCCGAGGATGCCAAACTTCAGCTGAGCAAGGACACCTAC 789 (G-->T) fv
oIG0036 AGGTGTCCTTGCTCAGCTGAAGTTTGGCATCCTCGGCC 789(G-->C) rev
oIG0037 ACCGGTTAATACTAGTAGCGGCCGC pSB1C3-RFC25_fw (AgeI, SpeI, NotI)
oIG0038 GCCGGCCATCTAGAAGCGGC pSB1C3-RFC25_rev (NgoMIV, XbaI)
oIG6017 GAGTGCCACCTGACGTCTAAGAAAC primerpBS1C3fw sequencing
oIG6018 CGCCTTTGAGTGAGCTGATACCGC primerpBS1C3rv sequencing
oIG0039 ATGATTTCTGGAATTCGCGGCCGCTTCTAGATGGCCGGCGCGCGTACGAAAAACAATTACGGGTC VP16 [25] for
oIG0040 TTTTGCCGGACTGCAGCGGCCGCTACTAGTATTAACCGGTCCCACCGTACTCGTCAATTCCAAGG VP16 [25] rev
oIG0041 ATGATTTCTGGAATTCGCGGCCGCTTCTAGATGGCCGGCATGGATGCTAAGTCACTAACTGCCTGG KRAB [25] for
oIG0042 TTTTGCCGGACTGCAGCGGCCGCTACTAGTATTAACCGGTCCAGAGATCATTCCTTGCCATTCCTTC KRAB [25] rev
oIG0043 TTCTGGAATTCGCGGCCGCTTCTAGATGGCCGGCCTGTGGAATGTGTGTCAGTTAGGGTG SV40 Promoter [25] fw
oIG0044 CGGACTGCAGCGGCCGCTACTAGTATTAACCGGTAGCTTTTTGCAAAAGCCTAGGCCTCC SV40 Promoter [25] rev
oIG0045 CCGGCGCAGGCTCCACCGGTTAATA Linker: Scar (Ala-Gly) - Ala-Gly-Ser - Scar (Thr-Gly) [25] fw
oIG0046 CTAGTATTAACCGGTGGAGCCTGCG Linker: Scar (Ala-Gly) - Ala-Gly-Ser - Scar (Thr-Gly) [25] fw
oIG0047 CCGGCTACCCATACGATGTTCCAGATTACGCTACCGGTTAATA HA-tag – fw primer
oIG0048 CTAGTATTAACCGGTAGCGTAATCTGGAACATCGTATGGGTAG HA-tag rev primer
oIG0049 TGATTTCTGGAATTCGCGGCCGCTTCTAGATGGCCGGCGTGCCTTCTAGTTGCCAGCC BGH-Terminator fv
oIG0050 CCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTAACCGGTCATAGAGCCCACCGCATCC BGH-Terminator rev
oIG0051 CCGGCGATAGTGCTGGTAGTGCTGGTAGTGCTGGTACCGGTTAATA DSAGSAGSAG-Linker fv
oIG0052 CTAGTATTAACCGGTACCAGCACTACCAGCACTACCAGCACTATCG DSAGSAGSAG-Linker rev
oIG0053 ATATCTTGTGGAAAGGACG colony pcr pIG3010 crRNA locus for
oIG0054 TTCTGCAGAATTGGCGCAC colony pcr pIG3010 crRNA locus rev
oIG0055 AACCGGTGCAGAATTGG
oIG0056 TTTTGCCGGACTGCAGCGGCCGCTACTAGTATTAACCGGTGCAGAATTGGCGCACGCGCTAG
oIG0057 AGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGTGCCTTCTAGTTGCCAGCC BGH (RFC 10) fwd
oIG0058 CCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTACATAGAGCCCACCGCATCC BGH (RFC 10) rev
oIG0059 AGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCTGTGGAATGTGTGTCAGTTAGGGTG SV40 (RFC 10) fwd
oIG0060 CCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTAAGCTTTTTGCAAAAGCCTAGGCCTCC SV40 (RFC 10) rev
oIG1000 CTAGCGCGTGCGCCAATTC pCBh-fwd_pX334a
oIG1001 GAAAGCTCTGAGGCACGATGGCGTCCACATCGTAGTCGGACAG hSpCsn1-H840A-rev_pX334a
oIG1002 TGTCCGACTACGATGTGGACGCCATCGTGCCTCAGAGCTTTCTG hSpCsn-H840A-fwd_pX334a
oIG1003 GTACGCGCGCGACCAGCACTACCAGCACTGTCGCCTCCCAGCTGAGAC VP16-linker-hSpCsn-rev_pX334a
oIG1004 TGGGAGGCGACAGTGCTGGTAGTGCTGGTCGCGCGCGTACGAAAAACAATTAC hSpCsn-linker-VP16-fwd_pKM018
oIG1005 GGAGCGGGTTGATGACGTCAGCGTTCGAATTGCGGCCGCGGCAGGATCATAATCAGCCATAC pX334a-SV40pA-rev_pKM018
oIG1007 AAACGGAAGGGCCTGAGTCCGAGCAGAAGAAGAAGT sticky end bsbI-EMXI Locus-sticky end bsbI-fwd_pX334a
oIG1008 TAAAACTTCTTCTTCTGCTCGGACTCAGGCCCTTCC sticky end bsbI-EMXI Locus-sticky end bsbI-rev_pX334a
oIG1009 CGATATCAGGAGGAAGGGCCTGAGTCCGAGCAGAAGAAGAAGGGCTCTCGCGACCTGCAGGG AatII-EcorV-emxIoperator-NruI-SbfI-NheI-fwd_pKM602
oIG1010 CTAGCCCTGCAGGTCGCGAGAGCCCTTCTTCTTCTGCTCGGACTCAGGCCCTTCCTCCTGATATCGACGT AatII-EcorV-emxIoperator-NruI-SbfI-NheI-rev_pKM602
oIG1011 AAACTTAAAAGTCGGCTGGTAGCGGGGAGGATCGGT human VEGFA locus 1_VZ-8_fwd_pKM600
oIG1012 TAAAACCGATCCTCCCCGCTACCAGCCGACTTTTAA human VEGFA locus 1_VZ-8_rev_pKM600
oIG1013 AAACGTGTGCAGACGGCAGTCACTAGGGGGCGCTGT human VEGFA locus 2_VZ-573_fwd_pKM600
oIG1014 TAAAACAGCGCCCCCTAGTGACTGCCGTCTGCACAC human VEGFA locus 2_VZ-573_rev_pKM600
oIG1015 AAACGACCTGCTTTTGGGGGTGACCGCCGGAGCGGT human VEGFA locus 3_VZ+434b_fwd_pKM600
oIG1016 TAAAACCGCTCCGGCGGTCACCCCCAAAAGCAGGTC human VEGFA locus 3_VZ+434b_rev_pKM600
oIG1017 AAACGTGAGTGTGTGCGTGTGGGGTTGAGGGCGTGT human VEGFA locus 4_VZ-475__fwd_pKM600
oIG1018 TAAAACACGCCCTCAACCCCACACGCACACACTCAC human VEGFA locus 4_VZ-475_rev_pKM600
oIG1019 CGATATCAGGATTAAAAGTCGGCTGGTAGCGGGGAGGATCGCGGCTCTCGCGACCTGCAGGG AatII-EcorV-VEGFA_VZ-8-NruI-SbfI-NheI-fwd
oIG1020 CTAGCCCTGCAGGTCGCGAGAGCCGCGATCCTCCCCGCTACCAGCCGACTTTTAATCCTGATATCGACGT AatII-EcorV-emxIoperator-NruI-SbfI-NheI-rev
oIG1021 CGATATCAGGAGTGTGCAGACGGCAGTCACTAGGGGGCGCTCGGCTCTCGCGACCTGCAGGG AatII-EcorV-VEGFA_ VZ-573-NruI-SbfI-NheI-fwd
oIG1022 CTAGCCCTGCAGGTCGCGAGAGCCGAGCGCCCCCTAGTGACTGCCGTCTGCACACTCCTGATATCGACGT AatII-EcorV-VEGFA_ VZ-573-NruI-SbfI-NheI-rev
oIG1023 CGATATCAGGAGACCTGCTTTTGGGGGTGACCGCCGGAGCGCGGCTCTCGCGACCTGCAGGG AatII-EcorV-VEGFA_ VZ+434b-NruI-SbfI-NheI-fwd
oIG1024 CTAGCCCTGCAGGTCGCGAGAGCCGCGCTCCGGCGGTCACCCCCAAAAGCAGGTCTCCTGATATCGACGT AatII-EcorV-VEGFA_ VZ+434b-NruI-SbfI-NheI-rev
oIG1025 CGATATCAGGAGTGAGTGTGTGCGTGTGGGGTTGAGGGCGTAGGCTCTCGCGACCTGCAGGG AatII-EcorV-VEGFA_ VZ-475-NruI-SbfI-NheI-fwd
oIG1026 CTAGCCCTGCAGGTCGCGAGAGCCTACGCCCTCAACCCCACACGCACACACTCACTCCTGATATCGACGT AatII-EcorV-VEGFA_ VZ-475-NruI-SbfI-NheI-rev
oIG1027 GTTCTCCTCCTCAACTG Sequencing Primer pKM602
oIG1028 GTAACTTGAAAGTATTTCGATTTCTTG DR-fwd_pX334a
oIG1029 GCGGCCGCAATTCGAACG H1Promotor-fwd_BBpX334a
oIG1030 CACAAGATATATAAAGCCAAGAAATC U6 Promotor-rev_BBpX334a
oIG2000 CAGACCCCGTAGAAAAGATCAAAGGATC Ori1-hU6-CAGenhancer-hSpCas9-fwd_pX334a
oIG2001 GAAAGCTCTGAGGCACGATAGCGTCCACATCGTAGTCGGAC hSpCas9-H840A-rev_pX334a
oIG2002 GTCCGACTACGATGTGGACGCTATCGTGCCTCAGAGCTTTC H840A-linkerGSAGSAG-KRAB-fwd_pX334a
oIG2003 CTTAGCATCCATGCCGGCGGAGCCTGCTGATCCGGGGCTGTCGCCTCCCAGC H840A-linkerGSAGSAG-KRAB-rev_pX334a
oIG2004 GACAGCCCCGGATCAGCAGGCTCCGCCGGCATGGATGCTAAGTCACTAACTGCCTGGTC linkerGSAGSAG-KRAB-bgHTerminator-fwd_pKM102
oIG2005 AGAAGGCACAGTCGAGGCTGATCGAAGCTTGGATCCTCACACCTTCC linkerGSAGSAG-KRAB-bgHTerminator-rev_pKM102/pX334a
oIG2006 GGAAGGTGTGAGGATCCAAGCTTCGATCAGCCTCGACTGTGCCTTCTAG bgHTerminator-PuroR/Backbone1-fwd_pKM102/pX334a
oIG2007 CACACCCGCCGCGCTTAATGCG bgHTerminator-PuroR/Backbone1-rev_pX334a
oIG2008 GCCCTGTAGCGGCGCATTAAGCG Backbone1-AmpR-Ori1-fwd_pX334a
oIG2009 GGATCTCAAGAAGATCCTTTGATCTTTTCTAC Backbone1-AmpR-Ori1-rev_pX334a
oIG2010 AAACGGAAGGGCCTGAGTCCGAGCAGAAGAAGAAGT sticky end BbsI-EMX1-sticky end BbsI-fwd_pX334
oIG2011 TAAAACTTCTTCTTCTGCTCGGACTCAGGCCCTTCC sticky end BbsI-EMX1-sticky end BbsI-rev_pX334
oIG2012 CTAGGGAAGGGCCTGAGTCCGAGCAGAAGAAGAAGGG sticky end NheI-EMX1-sticky end NheI-fwd_pKM006
oIG2013 CTAGCCCTTCTTCTTCTGCTCGGACTCAGGCCCTTCC sticky end NheI-EMX1-sticky end NheI-rev_pKM006
oIG2014 AATTGGAAGGGCCTGAGTCCGAGCAGAAGAAGAAGGG sticky end EcoRI-EMX1-sticky end EcoRI-fwd_pKM006
oIG2015 AATTCCCTTCTTCTTCTGCTCGGACTCAGGCCCTTCC sticky end EcoRI-EMX1-sticky end EcoRI-rev_pKM006
oIG2016 AAACGGATCTGCACCACCGGCAAGCTGCCCGTGCCCGT sticky end BbsI-pKM006 target_nt650-sticky end BbsI-fwd_pX334
oIG2017 TAAAACGGGCACGGGCAGCTTGCCGGTGGTGCAGATCC sticky end BbsI-pKM006 target_nt650-sticky end BbsI-rev_pX334
oIG2018 AAACGGCACGACTTCTTCAAGTCCGCCATGCCCGAGT sticky end BbsI-pKM006 target_nt750-sticky end BbsI-fwd_pX334
oIG2019 TAAAACTCGGGCATGGCGGACTTGAAGAAGTCGTGCC sticky end BbsI-pKM006 target_nt750-sticky end BbsI-rev_pX334
oIG2020 AAACGGCGACACCCTGGTGAACCGCATCGAGCTGAAGT sticky end BbsI-pKM006 target_nt850-sticky end BbsI-fwd_pX334
oIG2021 TAAAACTTCAGCTCGATGCGGTTCACCAGGGTGTCGCC sticky end BbsI-pKM006 target_nt850-sticky end BbsI-rev_pX334
oIG2022 AAACGGCGTCTATATCACCGCCGACAAGCAGAAGAAGT sticky end BbsI-pKM006 target_nt950-sticky end BbsI-fwd_pX334
oIG2023 TAAAACTTCTTCTGCTTGTCGGCGGTGATATAGACGCC sticky end BbsI-pKM006 target_nt950-sticky end BbsI-rev_pX334
oIG2024 AAACGGAGAGCTCGTTTAGTGAACCGTCAGATCGCCGT sticky end BbsI-pKM006 target_CMVmin1-sticky end BbsI-fwd_pX334
oIG2025 TAAAACGGCGATCTGACGGTTCACTAAACGAGCTCTCC sticky end BbsI-pKM006 target_CMVmin1-sticky end BbsI-rev_pX334
oIG2026 AAACGGCACGCTGTTTTGACCTCCATAGAAGACACCGT sticky end BbsI-pKM006 target_CMVmin2-sticky end BbsI-fwd_pX334
oIG2027 TAAAACGGTGTCTTCTATGGAGGTCAAAACAGCGTGCC sticky end BbsI-pKM006 target_CMVmin2-sticky end BbsI-rev_pX334
oIG2028 CCGGTGCCACCATGTACCCATAC hSpCas9-H840A-fwd_pX334a
oIG2029 CGTAATCTGGAACATCGTATGGGTACATG Ori1-hU6-CAGenhancer-hSpCas9-H840A-rev_pX334a
oIG2030 AAACCAAGGTCGGAGTACTGTCCTCCGACACTAGGT sticky end bsbI-UAS_Zebrafish_Locus_pos58-sticky end bsbI-fwd
oIG2031 TAAAACCTAGTGTCGGAGGACAGTACTCCGACCTTG sticky end bsbI-UAS_Zebrafish_Locus_pos58-sticky end bsbI-rev
oIG2032 AAACGAAATTCGGTCCATCCGCGCGCGCAGAGTGGT sticky end bsbI-Bmp2b_Zebrafish_Locus_pos55-sticky end bsbI-fwd
oIG2033 TAAAACCACTCTGCGCGCGCGGATGGACCGAATTTC sticky end bsbI-Bmp2b_Zebrafish_Locus_pos55-sticky end bsbI-rev
oIG2034 AAACAACTCCTCCATGCATATGTTAATGCGCCGCGT sticky end bsbI-Pouf51-VOX_Zebrafish_Locus_pos125-sticky end bsbI-fwd
oIG2035 TAAAACGCGGCGCATTAACATATGCATGGAGGAGTT sticky end bsbI-Pouf51-VOX_Zebrafish_Locus_pos125-sticky end bsbI-rev
oIG2036 TAATACGACTCACTATAGGGACGAAACACCGGGTTTTAGAGC crRNA with T7 site (forward)
oIG2037 CACGCGCTAGAAAAAGTTTTGGGACC crRNA (reverse)
oIG2038 TAATACGACTCACTATAGGGACCGGTGCCACCATGTACC Cas9 with T7 site and Kozak-sequence (forward)
oIG2039 AATTGCGGCCGCTCCCCAGC bGH Terminator (reverse)
oIG2040 GCAATAGCATCACAAATTTCACAAATAAAGC SV40 Terminator (reverse)
oIG2041 TAATACGACTCACTATAGGGGTTGGAACCATTCAAAACAGCATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTTGGCG tracrRNA with T7 site (forward)
oIG2042 CGCCAAAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATGCTGTTTTGAATGGTTCCAACCCCTATAGTGAGTCGTATTA tracrRNA (reverse)
oIG2043 AAACGATGCGGTTTTGGCAGTACATCAATGGGCGGT sticky end BbsI-CMV target of pIG6000-sticky end BbsI-fwd
oIG2044 TAAAACCGCCCATTGATGTACTGCCAAAACCGCATC sticky end BbsI-CMV target of pIG6000-sticky end BbsI-rev
oIG3001 GGCAGCGCCGGCTCCGGCAGCGCCTCCGGCATGGTGAGCAAGGGCGAGGAGG Linker1-mCherry_forw_pMZ333
oIG3002 TCTGGATCGAAGCTTGGGCTGCAGGTCGACTCTAGATCACTTGTACAGCTCGTCCATGCCGC mCherry-Bb_rev_pMZ333
oIG3003 TGTCTTTTATTTCAGGTCCCGGATCGAATTGCGGCCGCAGGAGGCGCCACCATGTACCCATACGATGTTCCAGATTACGCTGACAAGAAGTACAGCATCGGCCTG Bb-HA-Cas9_forw_pX334a
oIG3004 GCCGGAGGCGCTGCCGGAGCCGGCGCTGCCGTCGCCTCCCAGCTGAGACAG Cas9-Linker1_rev_pX334a
oIG3005 TGTCTTTTATTTCAGGTCCCGGATCGAATTGCGGCCGCAGGAGGCGCCACCATGTACCCATACGATGTTCCAGATTACGC Bb-HA(-NLS-Cas9)_forw_pMZ333
oIG3006 TCTGGATCGAAGCTTGGGCTGCAGGTCGACTCTAGATCAGCTGGCCTCCACTTTTCTCTTCTTTTTGGGGCTCTTGTACAGCTCGTCCATGCCGC mCherry-NLS-Bb_rev_pMZ333
oIG3007 TGTCTTTTATTTCAGGTCCCGGATCGAATTGCGGCCGCAGGAGGCGCCACCATGTACCCATACGATGTTCCAGATTACGCTATGAGAGCTGCCAACCTTTGGCCAAG Bb-HA-ERT2_forw_pMItom
oIG3008 GGACGCTTCGACCTTGCGCTTTTTCTTCGGCGAGACTGTGGCAGGGAAACCCTCTG ERT2-NLS(-Cas9)_rev_pMItom
oIG3009 GGGGAGGCAGAGGGTTTCCCTGCCACAGTCTCGCCGAAGAAAAAGCGCAAGGTC ERT2-NLS(-Cas9)_forw_pX334a
oIG3010 GCTGGCCTCCACTTTTCTCTTCTTTTTGGGGCTCTTGTACAGCTCGTCCATGCCGC mCherry-NLS3'_rev_pMZ333
oIG3011 AGCCCCAAAAAGAAGAGAAAAGTGGAGGCCAGCATGAGAGCTGCCAACCTTTGGCCAAG NLS3'-ERT2_forw_pMItom
oIG3012 TCTGGATCGAAGCTTGGGCTGCAGGTCGACTCTAGATCAGACTGTGGCAGGGAAACCCTCTG ERT2-Bb_rev_pMItom
oIG3013 AAACGATGATGAGGTTCTTGGCGGCTGTCTGTGCGT crRNA_SEAP(nt136-166)_forw
oIG3014 TAAAACGCACAGACAGCCGCCAAGAACCTCATCATC crRNA_SEAP(nt136-166)_rev
oIG3015 AAACCTGGCACATGTTTGTCTACATTGTATGTCTGT crRNA_SEAP(nt293-323)_forw
oIG3016 TAAAACAGACATACAATGTAGACAAACATGTGCCAG crRNA_SEAP(nt293-323)_rev
oIG4000 GATATGGCGGGGGAACAGGGG mCherry-fwd_pMZ700
oIG4001 GCCGCTGCCGGCGCCGCTGCCGGCGCTGCCCTTGTACAGCTCGTCCATGCCGC mCherry-rev_pMZ700/pMZ701
oIG4002 GGCAGCGCCGGCAGCGGCGCCGGCAGCGGCGACAAGAAGTACAGCATCGGCCTG cas9-fwd_pMZ1025
oIG4003 GTCTGGATCGAAGCTTGGGCTGCAGGTCGACTCTAGATTAGTCGCCTCCCAGCTGAGACAG cas9-rev_pMZ1025
oIG4004 GTTATTGGGGCTTTCTGTTTCTTGCAAATCC mCherry-fwd_pMU701
oIG4005 GTCTGGATCGAAGCTTGGGCTGCAGGTCGACTCTAGATTACACCTTTCTCTTCTTCTTGGGGCTGTC cas9(with NLS)-rev_pMZ1025
oIG4006 TCGAAGCTTGGGCTGCAGGTCGACTCTAGATTACACCTTTCTCTTCTTCTTGGGGCTGTCGCCTCCCAGCTGAGACAG cas9(with NLS)-rev_pIG4004 and pIG4005
oIG5000 TGCCTGAAATCACTTTTTTTCAGGTTGGACCGGTGCCACCTCGCGAATGTACCCATACGATGTTCCAGATTACG Backbone-Nru1-HA_for_pX334a
oIG5001 GGAGGCGGTGGCAGTGGTGGCGGAGGCAGTGGATCCATGGCCGACAAGCAGAAGAACGGC (GGGGS)3_Linker-BamH1-splitC_YFP_for_BBa_K323080
oIG5002 AAGTTAGTAGCTCCGCTTCCGCTGGCCTCCACCTTTCTCTTCTTCTTGGGGCTGTCGACCTTGTACAGCTCGTCCATGCCGAG P2A(first 20bp)-NLS-Sal1-splitC_YFP_rev_BBa_K323080
oIG5003 GGAGGCGGTGGCAGTGGTGGCGGAGGCAGTGGATCCGTGAGCAAGGGCGAGGAGCTG (GGGGS)3_Linker-BamH1-splitN_YFP_for_BBa_K323005
oIG5004 AAGTTAGTAGCTCCGCTTCCGCTGGCCTCCACCTTTCTCTTCTTCTTGGGGCTGTCGACGATGTTGTGGCGGATCTTGAAGTTCAC P2A(first20 bp)-NLS-Sal1-splitN_YFP_rev_BBa_K323005
oIG5007 GGAAGCGGAGCTACTAACTTCAGCCTGCTGAAGCAGGCTGGAGACGTGGAGGAGAACCCTGGACCTATGGTGAGCAAGGGCGAGGAGG P2A-mCherry_for_pMH206
oIG5008 CAACTAGAAGGCACAGTCGAGGCTGATCAGCGAGCTCTAGTCGCGATTACTTGTACAGCTCGTCCATGCCGC Backbone(pX334a)-Nru1-mCherry_rev_pMH206
oIG5009 CTAGAGCTCGCTGATCAGCCTCG forward - pX334a backbone amplification (Part1)
oIG5010 TCAAAAGAATAGCCCGAGATAGGGTTGAG reverse - pX334a backbone amplification (Part1)
oIG5011 CTTTAATAGTGGACTCTTGTTCCAAACTGGAAC forward - pX334a backbone amplification (Part2)
oIG5012 GGTGGCACCGGTCCAACCTG reverse - pX334a backbone amplification (Part2)
oIG5013 ACTGCCTCCGCCACCACTGCCACCGCCTCCGTCGCCTCCCAGCTGAGACAG (GGGGS)3_Linker-hCas9_rev_pX334a
oIG5014 GGAGGCGGTGGCAGTGGTGGCGGAGGCAGTGGATCCATGGAGAACACTGAAAACTCAGTGGATTC (GGGGS)3_Linker-BamH1-Caspase3_for_pMK31
oIG5015 AAGTTAGTAGCTCCGCTTCCGCTGGCCTCCACCTTTCTCTTCTTCTTGGGGCTGTCGACATGATGATGATGATGATGGTGATAAAAATAGAGTTC P2A(first 20bp)-NLS-Sal1-Caspase3_rev_pMK31
oIG5016 CTTAGCGGCAGCTTCCTTAGCGGCAGCTTCGTCGCCTCCCAGCTGAGACAG (EAAAK)3_Linker-hCas9_rev_pX334a
oIG5017 GAAGCTGCCGCTAAGGAAGCTGCCGCTAAGGGATCCATGGAGAACACTGAAAACTCAGTGGATTCAAAATC (EAAAK)3_Linker-BamH1-Caspase3_for_pMK31
oIG5018 GGAGGCGGTGGCAGTGGTGGCGGAGGCAGTGGATCCAGGCAAGCAGCAAAGTTGTCGAAGCC (GGGGS)3_Linker-BamH1-Casp9_for_pKJ42
oIG5019 AAGTTAGTAGCTCCGCTTCCGCTGGCCTCCACCTTTCTCTTCTTCTTGGGGCTGTCGACTGATGTTTTAAAGAAAAGTTTTTTCCGGAGG P2A(first 20bp)-NLS-sal1-Casp9(long)_rev_pKJ42
oIG5021 GAAGCTGCCGCTAAGGAAGCTGCCGCTAAGGGATCCAGGCAAGCAGCAAAGTTGTCGAAGCC (EAAAK)2-BamH1-Casp9_for_pKJ42
oIG5022 GTCTATATAAGCAGAGCTCTCTGGCATGAGCCCCAAGAAGAAGAGAAAGGTGGAGGCCAGCATGTCGAATTCTTATGACTCCTCCAGTATC Backbone(pcDNA_3.1)-NLS-GyrB-Caspase3_for_pMK31
oIG5023 GTCTATATAAGCAGAGCTCTCTGGCATGAGCCCCAAGAAGAAGAGAAAGGTGGAGGCCAGCAGGCAAGCAGCAAAGTTGTCGAAGC Backbone(pcDNA_3.1)-NLS-Caspase9-GyrB_for_pKJ42
oIG5024 AAGTTAGTAGCTCCGCTTCCGCTGGCCTCCACCTTTCTCTTCTTCTTGGGGCTGCCTTCATAGTGGAAGTGGTCTTCTTTG P2A(first 20bp)-NLS-Caspase9-GyrB_rev_pKJ42
oIG5025 ACTGCCTCCGCCACCACTGCCACCGCCTCCGCCTTCATAGTGGAAGTGGTCTTCTTTG (GGGGS)2_Linker-GyrB_rev_pMK31
oIG5026 ATGGCTGGCAACTAGAAGGCACAGTCGAGGTCACTTGTACAGCTCGTCCATGCCGC Backbone(pcDNA_3.1)-mCherry_rev_pMH206
oIG5027 CCTCGACTGTGCCTTCTAGTTGCC forward - pcDNA_3.1 backbone amplification (Part1)
oIG5028 CACTTTATGCTTCCGGCTCGTATGTTG reverse - pcDNA_3.1 backbone amplification (Part1)
oIG5029 CCACACAACATACGAGCCGGAAGC forward - pcDNA_3.1 backbone amplification (Part2)
oIG5030 GCCAGAGAGCTCTGCTTATATAGACC reverse - pcDNA_3.1 backbone amplification (Part2)
oIG5031 CAGGTTGGACCGGTGCCACCTCGCGAATGAGCCCCAAGAAGAAGAGAAAGGTGGAGGCCAGCGGATCCAGGCAAGCAGCAAAGTTGTCGAAGC Backbone(pX334a)-Nru1-NLS-BamH1-Caspase9(long)_for_pKJ42
oIG5032 ACTGCCTCCGCCACCACTGCCACCGCCTCCGTCGACTGATGTTTTAAAGAAAAGTTTTTTCCGGAGG (GGGGS)2_Linker_Sal1_Caspase9(long)_rev_pKJ42
oIG5033
oIG5034 GGAGGCGGTGGCAGTGGTGGCGGAGGCAGTGACAAGAAGTACAGCATCGGCCTGG (GGGGS)2_Linker-hCas9-NLS_for_pX334a
oIG5035 CTTCAGCAGGCTGAAGTTAGTAGCTCCGCTTCCAGCGTAATCTGGAACATCGTATGGGTACATGCTGGCCTCCACCTTTCTCTTCTTC P2A(first 33bp)-HA-hCas9-NLS_rev_pX334a
oIG6000 TCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCAGCTAGCTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTC pCMVfor-psB1C3
oIG6001 CGAGCGGTCCGCGGAACCAGCGCTACCGGACTGCCATTGCTCCGGCTGATCTGACGGTTCACTAAACCAGCTC pCMVrev-eGFP
oIG6002 AGCCGGAGCAATGGCAGTCCGGTAGCGCTGGTTCCGCGGACCGCTCGGCCACCATGGTGAGCAAGGGC eGFPfor-CMV
oIG6003 GGACGCTTCGACCTTGCGCTTTTTCTTCGGCGAGGGATCCGACCCGTCGACGCCCTTGTACAGCTCGTCCATGC eGFPrev-Sal1-Bamh1-NLS
oIG6004 TCGCCGAAGAAAAAGCGCAAGGTCGAAGCGTCCTAAAGATCTATAATCAGCCATACCACATTTGTAGAGGTTTTAC terminatorfor-NLS
oIG6005 GCCGGACTGCAGCGGCCGCTACTAGTAAGGTACCGTAAGCTTTAGCTCTGAATGCATGCGTATTGCTAATGGGTCTAATGCAGTTAAGATACATTGATGAGTTTGGACAAACCAC terminatorrev-Nru1-Hind3-pSB1C3-Spe1-Not1-Pst1
oIG6006 AAACCCCTCGCCCTCGCCGGACACGCTGAACTTGGT eGFPfw
oIG6007 TAAAACCAAGTTCAGCGTGTCCGGCGAGGGCGAGGG eGFPrv
oIG6008 AAACGGCGGGCCATTTACCGTAAGTTATGTAACGGT CMVfw
oIG6009 TAAAACCGTTACATAACTTACGGTAAATGGCCCGCC CMVrv
oIG6010 AAACTTATGTTTCAGGTTCAGGGGGAGGTGTGGGGT sv40 terminatorfw
oIG6011 TAAAACCCCACACCTCCCCCTGAACCTGAAACATAA sv 40 terminatorrv
oIG6012 GGACGCTTCGACCTTGCGCTTTTTCTTCGGCGAGGGATCCGACCCGTCGACGCCTTACTTGTACAGCTCGTCCATGCCG eCFPrv
oIG605neu GTTCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTAAGGTACCGTAAGCTTTAGCTCTGAATGCATGCGTATTGCTAATGGGTCTAATGCAGTTAAGATACATTGATGAGTTTGGACAAACCAC rv primer für den terminator mit mehr basen overlapp
oIG6015 ATGTACACTAAGTCTAGCTAGACTAGCTGGTACCGACTAGTAGCGGCCGCTGCAGTC
oIG6016 GCTATAGCTAAGCGCGTCGAGTCGAGCGAAGCTTATCTAGAAGCGGCCGCGAATTCCAG
oIG6017 GAGTGCCACCTGACGTCTAAGAAAC primer für pSB1C3 fw
oIG6018 CGCCTTTGAGTGAGCTGATACCGC primer für pSB1C3 rv
oig6020 TCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCAGCTAGCCGATGTACGGGCCAGATATACGCG CMV fw
oig6021 CGAGCGGTCCGCGGAACCAGCGCTACCGGACTGCCATTGCTCCGGCTAATTTCGATAAGCCAGTAAGCAGTGGGTTC CMV rv
oig6022 TCGCCGAAGAAAAAGCGCAAGGTCGAAGCGTCCTAAAGATCTCGACTGTGCCTTCTAGTTGCCAGC bgh terminator fw
oig6023 GCCGGACTGCAGCGGCCGCTACTAGTAAGGTACCGTAAGCTTTAGCTCTGAATGCATGCGTATTGCTAATGGGTCTAATGCACCATAGAGCCCACCGCATCCC bgh terminator rv
oig6024 AGCCGGAGCAATGGCAGTCCGGTAGCGCTGGTTCCGCGGACCGCTCGGCCACCATGGCCACAACCATGGTGAGCAAGG eCFP fw
oig6025 GGACGCTTCGACCTTGCGCTTTTTCTTCGGCGAGGGATCCGACCCGTCGACGCCCTTGTACAGCTCGTCCATGCCGAG eCFP rv
oig6030 AAACGTTCAAGTCGCACTCGCCGCTCCCGCTCCCGT AvGFP fw beginning
oig6031 TAAAACGGGAGCGGGAGCGGCGAGTGCGACTTGAAC AvGFP rv beginning
oig6032 AAACGTAACTGCGTTTACCCGCCATCCGCACATGGT target CMV promoter before tata fw
oig6033 TAAAACCATGTGCGGATGGCGGGTAAACGCAGTTAC target CMV promoter before tata rv
oig6034 AAACAGTCTAGTCGGCCTCGTTACCGTCAGGCCAGT target CMV behind tata fw
oig6035 TAAAACTGGCCTGACGGTAACGAGGCCGACTAGACT target CMV behind tata rv
oig6036 AAACAGATCAACGGTCGGTAGACAACAAACGGGGGT target bgh terminator beginning fw
oig6037 TAAAACCCCCGTTTGTTGTCTACCGACCGTTGATCT target bgh terminator beginning rv
oig6038 AAACGTTCAAGTCGCACAGGCCGCTCCCGCTCCCGT target eCFP fw
oig6039 TAAAACGGGAGCGGGAGCGGCCTGTGCGACTTGAAC target eCFP rv
oIG8002 GCCGGCGGAGCCTGCTGATCCGGGGCTGTCGCCTCCCAGC Cas9_GSAGSAG_rev
oIG8005 GGACGCTTCGACCTTGCGCTTTTTC NLS1_pX334_rev
oIG8006 GGATCAGCAGGCTCCGCCGGCGACAAGAAGTACAGCATCGGCCTG Cas9_GSAGSAG_fw
oIG8007 GGTGCTGGTATGACAAG G9a_seq_fw_pGEX2T
oIG8008 GATGTTGCCATAGTAACGG G9a_seq_rev_pGEX2T
oIG8009 GACAGCCCCGGATCAGCAGGCTCCGCCGGCGAGAATGTACCCATCCCCTGTGTC G9a(mm)_C-term_GSAGSAG_fw
oIG8010 GGATCCTCACACCTTCCGCTTTTTCTTGGGGGTGTTGATGGGGGGCAGGGA G9a(mm)_C-term_NLS_bgh_rev
oIG8011 AAGCGCAAGGTCGAAGCGTCCGAGAATGTACCCATCCCCTGTGTC G9a(mm)_N-term_NLS_fw
oIG8012 GCCGGCGGAGCCTGCTGATCCGGTGTTGATGGGGGGCAGGGA G9a(mm)_N-term_GSAGSAG_rev
oIG8013 GCCCGTTACTATGGCAACATCAGCCGATTCATTAACAAGCTGTGTGACCCCAACATCATCCCTGTCCGGGTTTTCATGCTG dG9a(mm)_H->K_fw
oIG8014 CAGCATGAAAACCCGGACAGGGATGATGTTGGGGTCACACAGCTTGTTAATGAATCGGCTGATGTTGCCATAGTAACGGGC dG9a(mm)_H->K_rev
oIG8015 GAATTCGCGGCCGCTTCTAGATGGCCGGCGAGAATGTACCCATCCCCTGTGTC _G9a_fw_std
oIG8016 CTGCAGCGGCCGCTACTAGTATTAACCGGTGGTGTTGATGGGGGGCAGGGA G9a_rev_std
oIG8017 GCAGTCATCCACACACGTGCAGTGCTGGAGATGGGTGATGTTGCGGTCGA G9a_1stsite_std_rev
oIG8018 CAGCACTGCACGTGTGTGGATG G9a_1stsite_std_fw
oIG8019 GCCCATCTTGGCAGTCCGGTAGAGCTGGAGCCGTACCTTGATGCCGCTCT G9a_2ndsite_std_rev
oIG8020 CAGCTCTACCGGACTGCCAAGA G9a_2ndsite_std_fw
oIG8021 CCCAAGAAAAAGCGGAAGGTGTGATC phyB hole vector for
oIG8022 GCGGCTGTACGCGGAACCAG phyB hole vector rev
oIG8023 GCTGGTAGTGCTGGTTCCGCGTACAGCCGCGAGAATGTACCCATCCCCTGTGTC phyB(overhang)_g9a for
oIG8024 CTCTAGATCACACCTTCCGCTTTTTCTTGGGGGTGTTGATGGGGGGCAGGGA phyB(overhang)_g9a rev
oIG9100 TCATATCCGCGGCATGTACCCATACGATGTTCCAGATTACG cas9 fw incl. HA tag and NLS
oIG9191 GATATTGGTACCCATGCCTGCTATTCTCTTCCCAATCCTC cas9 rv incl. Bgh terminator
oIG4100F GCTAGCTAGTTATTAATAGTAATCAATTACGGG wird nachgetragen
oIG4100R GAATTCGATCTGACGGTTCACTAAAC
oIG4101F ATGTACCCATACGATGTTCCAG
oIG4101R GGGGCTGTCGCCTCCCAG
oIG4102F GACACGGATCGACCTGTCTCAGCTGGGAGGCGACAGCCCCCCGAAGAAAAAGCGCAAGGTCGCGCTCGATAGTGCTGGTAG
oIG4102R TCTTCGGCGAAGCGTAATCTGGAACATCGTATGGGTACATGGTGGGCGGCCGCAATTC
oIG4103F ATGGATGCTAAGTCACTAACTGCC
oIG4103R TCACACCTTCCGCTTTTTCT
oIG4104F AAGGAATGATCTCTGGCCCAAGAAAAAGCGGAAGGTGTGATCTAGAGTCGACCTGCAGCC
oIG4104R CCAGTGTCCGGGACCAGGCAGTTAGTGACTTAGCATCCATGCGGCTGTACGCGGAACCAG
oIG4105F GACACGGATCGACCTGTCTCAGCTGGGAGGCGACAGCCCCCCGAAGAAAAAGCGCAAGGTCGGTACCGGCGCCGGCG
oIG4105R TCTTCGGCGAAGCGTAATCTGGAACATCGTATGGGTACATGGTGGGAATTCGCGGCC
oIG4106F AAGGAATGATCTCTGGCCCAAGAAAAAGCGGAAGGTGTGAGGGGCGCGAGGATCCTCTAG
oIG4106R CCAGTGTCCGGGACCAGGCAGTTAGTGACTTAGCATCCATCGCGCCGGCGCCCGCAG
oIG4107F GTCTCAGCTGGGAGGCGACAGCCCCCCGAAGAAAAAGCGCAAGGTCGGTAGTGCTGGTAGTGCTGGTAGTGCTGGTATGAAGATGGACAAAAAGACTATAGTT
oIG4107R TGCTGCTCCGATCATGATCTG
oIG4108F TTCAAGAACCCGTGAAGCACAGATCATGATCGGAGCAGCATGATCTAGAGTCGACCTGCAGC
oIG4108R TCTTCGGCGAAGCGTAATCTGGAACATCGTATGGGTACATGGTGGGCGGCCGCAATTC
oIG4109F GATAGTGCTGGTAGTGCTGGT
oIG4109R GGTGGGCGGCCGCAATTC
oIG4110F CTTTTATTTCAGGTCCCGGATCGAATTGCGGCCGCCCACCATGAATGGAGCTATAGGAGGTGAC
oIG4110R TGTACGCGGAACCAGCACTACCAGCACTACCAGCACTATCATGAATATAATCCGTTTTCTCCAATTCCT
oIG4111F AAGGAATGATCTCTGGCCCAAGAAAAAGCGGAAGGTGTGATCTAGAGTCGACCTGCAGCC
oIG4111R GGTGGGCGGCCGCAATTC
oIG4112R AGGCAGTTAGTGACTTAGCATCCATGCGGCTGTACGCGGAACCAGCACTACCAGCACTACCAGCACTATCATGAATATAATCCGTTTTCTCCAATTCCT
crRNA tetO 1 F TAAAACATCAGTGATAGAGAAAAGTGAAAGTCGAGT
crRNA tetO 1 R AAACACTCGACTTTCACTTTTCTCTATCACTGATGT
crRNA tetO 2 F TAAAACCTCCCTATCAGTGATAGAGAAAAGTGAAAG
crRNA tetO 2 R AAACCTTTCACTTTTCTCTATCACTGATAGGGAGGT