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Team:UniSalento Lecce/Notebook
From 2013.igem.org
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"date":"1380268800000", /*27 settembre*/ | "date":"1380268800000", /*27 settembre*/ | ||
"type":"demo", | "type":"demo", | ||
| - | "title":"", | + | "title":"PLATES RESULTS: no growth.<br>Boiling prep of 10, 15, 15pLac, nikR + control gel (loading order: L; 10 A; 10 B; 10 C; 10 D; nikR 1; nikR 2; 15 A; 15 B; 15 C; 15 D; 15 E; 15pLac A; 15pLac B; 15 pLac C; 15pLac D; 15 pLac E; CTRL PSB1C3).<br>RESULTS: 15pLac 5 is a good sample, so proceed with digestion and PCR. For the other samples we need new inocula. BL21 inocula and growth (2h) fluorescent decay assay with constructs 1+4 and 1+5 (CTRL, + Ni, + IPTG, + Ni + IPTG).<br>RESULTS: published on the Register (Team Parts).", |
"description":" ", | "description":" ", | ||
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"date":"1380182400000", /*26 settembre*/ | "date":"1380182400000", /*26 settembre*/ | ||
"type":"demo", | "type":"demo", | ||
| - | "title":"", | + | "title":"PLATES RESULTS: No growth observed on NikR and 1+3 plates.<br>Remake the cloning for these samples.<br>Inocula for the other plates grown and preinocula for the two BL21 samples.", |
"description":" ", | "description":" ", | ||
"url":"#" | "url":"#" | ||
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"date":"1380096000000", /*25 settembre*/ | "date":"1380096000000", /*25 settembre*/ | ||
"type":"demo", | "type":"demo", | ||
| - | "title":"", | + | "title":"PLATES RESULTS: no growth observed.<br>Control digestion (single and double) and PCR of: 12.3, 12.4, 12.5, 1+5 A, 1+5 B, 1+5 D, 1+5 E, nikR 1, nikR 2.<br>Control gel (loading order digestions: L; nikR 1; nikR 2; 12.3; 12.4; 12.5; 1+5 A (Eco + Pst); 1+5 A; 1+5 B; 1+5 D; 1+5 E (Eco); loading order PCR: 12.3; 12.4; 12.5; 1+5 A; 1+5 B; 1+5 D; 1+5 E; nikR 1; nikR 2).<br>Rebuild the constructs 1+3 and nikR.<br>Digestion and ligation: 6 Amp C ES + 1 XP + PSB1C3 EP; 14.3 XP + B0034 ES + PSB1K3 EP; 14.3 XP + J04500 ES + PSB1A3 EP; nikR EP + PSB1C3 EP; 1 ES + 3 XP + PSB1C3 EP.<br>Transformation of DH5α cells and plate of 10, 15, 15-pLac, NikR and 1+3 in PSB1C3, BL21 1+5 and BL21 1+4 in LB agar + IPTG 1mM + related antibiotics.", |
"description":" ", | "description":" ", | ||
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"date":"1380009600000", /*24 settembre*/ | "date":"1380009600000", /*24 settembre*/ | ||
"type":"demo", | "type":"demo", | ||
| - | "title":"", | + | "title":"Ligations of the following constructs: 6 Amp C + 1 in PSB1C3 (construct n.10), 14.3 + B0034 in PSB1K3 (construct n.15), 14.3 + pLac-RBS (J04500) in PSB1A3 (pLac-RBS + construct n.15) 6 Amp C digested with Eco and Spe, 1 with Xba and Pst; 14.3 digested with Xba and Pst, B0034 with Eco and Spe; pLac-RBS (J04500) with Eco and Spe, 14.3 with Xba and Pst.<br>Transformation and plate on LB agar + IPTG 1mM + related antibiotics.<br>Boiling prep of the yesterday’s inocula + control gel (loading order: M; nikR 1÷5; 12 1÷5; 1+5 A÷E; 1+3 A÷E).", |
"description":" ", | "description":" ", | ||
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"date":"1379923200000", /*23 settembre*/ | "date":"1379923200000", /*23 settembre*/ | ||
"type":"demo", | "type":"demo", | ||
| - | "title":"", | + | "title":"Digestion and PCR to control the last boiling preps (2A, 3.1, 4.2, 14.3, pUreA1, Hpn1, 1+4 A, 1+4 C, 12.2, 1+5 A, 6 Amp C, 6 Kan 2ul C, 6 Kan 5ul A, 4.3, nikR A) + control gel (loading order digestions: L; 2A; 3.1; 4.3; 4.2; 12.2; 14.3; pUreA 1; Hpn 1; NikR A; 6 Amp C; 6 Kan 2ul C; 6 Kan 5ul A; 1+4 C; 1+5 A; 1+4 A. loading order PCR: L; 2A; 3.1; 4.2; 14.3; pUreA 1; Hpn 1; 1+4 A; 1+4 C; 12.2; 1+5 A; 6 AmpC; 6 Kan 2 ul C; 6 Kan 5 ul A; 4.3; nikR A).<br>RESULTS: next inocula: 12, nikR in C; 1+5; 1+3 (5 tubes per sample). ", |
"description":" ", | "description":" ", | ||
"url":"#" | "url":"#" | ||
Revision as of 08:29, 1 October 2013
Lab Notebook
Here you can follow our experiments and results since the beginning of our work. Click on a day in the calendar to find out how we spent those neverending days in the lab! (We thank Emanuele for keeping an up-to-date journal!)
