Team:TU-Munich/Project/Phytoremediation

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Phytoremediation

What it´s all about

As industrialization reached ever more parts of the globe throughout the last decades the standard of living increased drastically around the world. However nothing comes without a price and the toll not only we but our children and grandchildren have to pay for our luxury is a polluted environment which will someday cloud all the great modern comforts of our time.
Today a great variety of science and technology is eager to protect the environment from further harm and reverse the damage already done. Of these technologies bioremediation, and phytoremediation in particular, seems to be the most promising one as it tries to help nature help itself. Bioremediation was defined as "the process of judiciously exploiting biological processes to minimize an unwanted environmental impact; usually it is the removal of a contaminant form the biosphere." http://onlinelibrary.wiley.com/doi/10.1002/0471238961.0209151816180914.a01/abstract Prince, 2000 Phytoremediation is the concept of removing these pollutans either directly by plants themselves or by specialized bacteria living in a symbiosis with plants. There is a multitude of different pollutants that are primary targets for remediation such as Polychlorinated biphenyls (PCBs), insecticides such as dichlorodiphenyltrichloroethane (DDT), heavy metals such as cadmium or mercury or pharmaceutical products such as diclofenac or ethynyl estradiol .

Phytoremediation vs. Bioremediation

So why did we choose Phytoremediation over the approach of classical Bioremediation using bacteria like so many iGEM-teams do?
For this there are several reasons. For one we thought that the overall applicability of plants is much higher than of bacteria. Provided a sufficient saftey mechanism transgenic plants can be deployed almost everywhere. All they need is light and they will sustain themselves. Bacteria on the other hand are much harder to contain and are generally more expensive than plant systems.

Costs of different techniques for soil remediation
Type of treatment	Range of costs, $/ton
Phytoremediation	10-35
In situ bioremediation	50-150
Soil venting	20-220
Indirect thermal treatment	120-300
Soil washing	80-200
Solidification/stabilization	240-340
Solvent extraction	360-460
Incineration	200-1500

Considering all these factors we decided that Phytoremediation was the way to go. And thanks to all the fantastic work of other iGEM-teams in previous years we had the possibility to choose from a wide variety of existing biodegradation bricks!
Our main goal in this project was to use the excellent existing bricks and build up on the work of previous teams but by establishing a completely new chassis we wanted to go one step further.

Previous Work in iGEM and the Choice of a suitable Chassis

As we established our idea we investigated several remediation projects done by previous teams to look for ways to improve them or even come up with a totally new approach. Looking through interesting projects like those of [http://2012.igem-bielefeld.de/index.php Bielefeld 2012], Imperial College London 2010 and of course the algae project of Bilkent UNAM Turkey, we noticed that there have only been very few phytoremediation projects in the iGEM competition. We thought there could be a more suitable chassis for remediation and stumbled upon the recently established model organism Physcomitrella patens. Since noone in our team had ever worked with moss (or with plants for that matter) we had a lot of research to do. Thankfully [http://www.plant-biotech.net/ Prof. Reski´s homepage] provided the necessary papers, methods and details. Having looked into it, we were conviced that this plant is what we have been looking for and that it would be great to introduce it into iGEM.

**Previous remediation projects:**
Team	Year	Organism	Description
Peking University	2010	E.coli	Heavy metal decontamination of aquatic environments via binding proteins
UT Dallas	2010	E.coli	Establishing E.coli as a biosensor for environmental pollutants using fluorescence
METU Turkey	2010	E.coli	Designing a biosensor to detect CO as a dangerous pollutant
USeoul Korea	2010	E.coli	Using fluorescence proteins to detect various heavy metals in water
TU Delft	2010	E.coli	Enabling hydrocarbon degradation in aqueous environments
Michigan	2009	E.coli	Sensing and degrading toluol
UQ Australia	2009	E.coli	Uptake and reduction of mercury in water
Cornell University	2009	B.subtilis	Creating a cadmium sensor

Seeing all those great projects we decided not only to improve biodegredation by looking at the actual degredation processes but more importantly by establishing a wholly new chassis to the iGEM competition and to phytoremediation in general.

Why Physco, why?

So why exactly did we choose Physcomitrella patens as the basis for our remediation project? One big point was that we wanted to make our filter system available for everyone and bacteria just didn´t do the trick for us: Harder to contol, harder to grow and harder to implement. We wanted something that could be deployed in a few simple steps and would be self-sustainable and self-renewing. Short, we wanted an autotrophic organism which could be easily established in an aquatic environment. Not only does our moss provide us with these features as it only needs water, light and carbon dioxide to grow and can be grown on soil as well as in water but it can also be easily cultivated in bioreactors which gives us the possibility to envision industrial scale waste-water treatment based on our system.

Previous Work (some examples)

MIT 2007 https://2007.igem.org/MIT

--> Filtering Mercury in E.coli

Bilkent UNAM Turkey (https://2011.igem.org/Team:Bilkent_UNAM_Turkey/Project_Description)

--> Degrading TNT in Algae

Bielefeld 2012 (Laccase)

--> Degrading Hormones in different organisms -> Cooperation with LMU

Collaboration with Paris-Saclay and Dundee

The advatages of Physcomitrella patens as a chassis include:

water as its natural environment
autotrophic
easy handling

Constructed Wetlands as a large-scale application of Phytoremediation

In the last decades Phytoremediation made the transition from a solely scientific approach to an industrial scale water treatment technique. Today large amounts of water are treated through constructed wetlands as it poses one of the simplest and most cost-effective ways to treat sewage-waters either on their own or in symbiosis with a traditional treatment plant.

How do they work and why do we need them?

Natural wetlands, as well as constructed ones, are systems in which plants and microorganisms form complex biotopes capable of filtering, degrading or inactivating various substances in the water. The wetland, slowing the speed of the passing water, can trap suspended solids in the vegetation which also accumulates pollutants by absorbing them. As these wetlands are a perfect habitat for microorganisms various degredation processes take place ridding the water from a variety of pollutants.

Higher environmental standards in first world countries as well as the growing industrialization have led to a skyrocketing demand in sewage water treatment. The capacities of traditional treatment systems have shown to be ineffective and too expensive. Constructed wetlands pose a real alternative to conventional technologies as they are not only reliable and cost-effective but the most natural and environmental friendly way of cleaning water. They leave no waste behind, are self-sustaining and have no demand for artificial energy. Other than most other environmental measurements constructed wetlands are very attractive for businesses as they are cheap alternatives to existing systems rather than costly additions.

References:

http://onlinelibrary.wiley.com/doi/10.1002/0471238961.0209151816180914.a01/abstract Prince, 2000 Prince, R. C. (2000). Bioremediation. Kirk-Othmer Encyclopedia of Chemical Technology.

@@ Line 109: / Line 109: @@
 ===Why ''Physco'', why?===
+So why exactly did we choose ''Physcomitrella patens'' as the basis for our remediation project? One big point was that we wanted to make our filter system available for everyone and bacteria just didn´t do the trick for us: Harder to contol, harder to grow and harder to implement. We wanted something that could be deployed in a few simple steps and would be self-sustainable and self-renewing. Short, we wanted an autotrophic organism which could be easily established in an aquatic environment. Not only does our moss provide us with these features as it only needs water, light and carbon dioxide to grow and can be grown on soil as well as in water but it can also be easily cultivated in bioreactors which gives us the possibility to envision industrial scale waste-water treatment based on our system.
 Previous Work (some examples)