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Team:Freiburg/Highlights
From 2013.igem.org
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uniBAss - Binding Assay</p> | uniBAss - Binding Assay</p> | ||
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| - | We developed an ELISA based method. With this method we can quantify the binding efficiency of our proteins. We called this binding assay uniBAss. | + | We developed an ELISA based method. With this method we can quantify the binding efficiency of our proteins. We called this binding assay uniBAss. This is a powerful tool for the characterizing the modified dCas9 by assessing it´s DNA binding capacity. |
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We developed an ELISA based method. With this method we can quantify the <b>binding efficiency </b>of our proteins. We called this binding assay <b>uniBAss</b>. It is | We developed an ELISA based method. With this method we can quantify the <b>binding efficiency </b>of our proteins. We called this binding assay <b>uniBAss</b>. It is | ||
| - | a powerful tool for | + | a powerful tool for characterizing the modified dCas9 by assessing it´s DNA binding capacity. |
</p> <p> | </p> <p> | ||
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Revision as of 13:09, 2 October 2013
HIGHLIGHTS
Discover a complete new breakthrough in the way you regulate your genes and feel the fascination of simple complexity.
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Get the uniCAS toolkit and be part of a fast growing new community of next generation scientific researchers. Discover the great possibilities of uniCAS Toolkit right below!
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6 Reasons for loving the uniCAS toolkit All you need to realize your personal experiments are 6 smart amazingly easy to use tools that come with the uniCAS toolkit. Just use our custom-tailored uniTOOL to generate individual manuals. Best of all: Its all open source and non commercial! |
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Cas9 makes it possible!
First derived from bacterial immune system this wonderful enzyme is a real multifunctional talent. Specific targeting of DNA has never been easier!
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Activation The activation domain VP16 is able to activate transcription of genes. |
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Repression The fusion of the transcriptional repressor domain KRAB leads to synthetic repression of gene expression. |
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Chromatin modification (Repression) Specific chromatin modification was achieved by fusing a histone methyltransferase G9a to dCas9. With this protein we are able to specifically repress endogenous gene expression. |
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uniBAss - Binding Assay We developed an ELISA based method. With this method we can quantify the binding efficiency of our proteins. We called this binding assay uniBAss. This is a powerful tool for the characterizing the modified dCas9 by assessing it´s DNA binding capacity. |
