* Bio-system 1: pCpxR promoter activates GFP expression. Will produce the green fluorescent protein on membrane stress.
+
* Silica Binding Peptide: A short amino acid sequence that binds silica with high affinity.
-
* Bio-system 2: Constitutive production of the Ice nucleation protein with a silica binding domain at the c-terminus.
+
* Bio-system 1: CpxR promoter controlling GFP expression. Will produce the green fluorescent protein following exposure to hydrophobic membrane stress.
-
* Bio-system 3: integration of 1 and 2, INP Si4is constitutively produced but the fluorescent protein is under control of pCpxR promoter. When bound to silica beads will fluoresce
+
* Bio-system 2: INP.Si4 is produced constitutively with GFP production under control of CpxR promoter. The system is designed to bind with high affinity to silica macro-structures and produce a fluorescent response when it does.
-
* Also planning on having the silica binding peptide submitted as its own part for others to use for their own purposes.
Please check back here frequently for a full list of the parts we've developed, submitted to the [http://partsregistry.org Registry] and have had approved. We will also endeavour to include our characeterisation protocols, although we are currently collaborating with Purdue to decide exactly what these should be.
Note to Team:proper part documentation is to describe and define a part such that it can be used without a need to refer to the primary literature. The next iGEM team should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more
Silica Binding Peptide: A short amino acid sequence that binds silica with high affinity.
Bio-system 1: CpxR promoter controlling GFP expression. Will produce the green fluorescent protein following exposure to hydrophobic membrane stress.
Bio-system 2: INP.Si4 is produced constitutively with GFP production under control of CpxR promoter. The system is designed to bind with high affinity to silica macro-structures and produce a fluorescent response when it does.
Submitted
A full listing of all parts Leeds iGEM 2013 have submitted to the Registry, and their status.
<groupparts>iGEM013 Leeds</groupparts>
"
