Team:Leeds/Parts

Note to Team: proper part documentation is to describe and define a part such that it can be used without a need to refer to the primary literature. The next iGEM team should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more

Currently Working On

• Silica Binding Peptide (Si4): A short amino acid sequence that binds silica with high affinity.
• Bio-system 1: CpxR promoter controlling GFP expression. Will produce the green fluorescent protein following exposure to hydrophobic membrane stress. Extensive experimental data has been acquired about the response of the cpxR promoter to various forms of physical and chemical stresses, this can be found in the biosystem 1 section of the Lab Results page.
• Bio-system 2: INP.Si4 is produced constitutively with GFP production under control of CpxR promoter. The system is designed to bind with high affinity to silica macro-structures and produce a fluorescent response when it does. Experimental data has been gathered for this part in unison with the silica binding peptide. Problems with expression meant that the data is not as conclusive as predicted but indicates that Si4 does bind silica with a degree of affinity.

Submitted

A full listing of all parts Leeds iGEM 2013 have submitted to the Registry, and their status.
<groupparts>iGEM013 Leeds</groupparts>