"
Team:INSA Toulouse/contenu/lab practice/results/red sensor
From 2013.igem.org
Mesnageclem (Talk | contribs) (Created page with "{{:Team:INSA_Toulouse/template/header}} {{:Team:INSA_Toulouse/template/sidebar}} <!--Contenu*/ /**********/--> <html> <!--- →open Sans : fonnt Google: --> <link href='htt...") |
Mesnageclem (Talk | contribs) |
||
| Line 153: | Line 153: | ||
<h1 class="title1">Results</h1> | <h1 class="title1">Results</h1> | ||
| - | <h2 class="title2"> | + | <h2 class="title2">Red Light Sensor Characterization</h2> |
Revision as of 18:57, 3 October 2013
Results
Red Light Sensor Characterization
XOR1
In vitro
Description des conditions d'expérimentation
In vitro recombinase characterization protocol
Back to the top
In vivo
Description des conditions d'expérimentation
In vitro recombinase characterization protocol
Back to the top
AND1
In vitro
Description des conditions d'expérimentation
In vitro recombinase characterization protocol
Back to the top
In vivo
Description des conditions d'expérimentation
In vitro recombinase characterization protocol
Back to the top
Red Light Sensor
In vitro
Description des conditions d'expérimentation
In vitro recombinase characterization protocol
Back to the top
In vivo
Description des conditions d'expérimentation
In vitro recombinase characterization protocol
Back to the top
Blue Light Sensor
In vitro
Description des conditions d'expérimentation
In vitro recombinase characterization protocol
Back to the top
In vivo
Description des conditions d'expérimentation
In vitro recombinase characterization protocol
Back to the top
Pol T7
In vitro
Description des conditions d'expérimentation
In vitro recombinase characterization protocol
Back to the top
In vivo
Description des conditions d'expérimentation
In vitro recombinase characterization protocol
Back to the top
General inductor
In vivo
The couple TetR-pTet system construction is a first step to characterize the entire system. A constitutive promoter (BBa_J23116) was assemble to tetR and then assemble to pTet-rbs-RFP-term (Bba_I13521). pTet is a regulator constitutively ON that expresses the mRFP1 protein. TetR is an inducer that binds to pTet promoter and thus, represses expression the downstream system. Supply of tetracycline or its analog aTc (anhydrotetracycline) is known to bind to tetR and invert the operation (inhibits expression of mRFP in this case).
After 18 hours, clones containing this assembly (BBa_J23116-TetR-pTet-RFP) show a leaky basal expression of mRFP. We suppose that the promoter was too weak to express TetR in large quantity. Assembly of a stronger promoter could improve the system and lock the response to an ON/OFF response.
Besides, an experience was done to analyze the effect of the aTc inducer. Result show a visible induction of the red fluorescent protein expression by addition of aTC (60 ng/mL).
Back to the top
