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Team:Tuebingen/Notebook/Protocols
From 2013.igem.org
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<li><a href="/Team:Tuebingen/Notebook/Protocols/sc-dropout">SC DropOut</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/sc-dropout">SC DropOut</a></li> | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/onestep">ONE-STEP buffer</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/onestep">ONE-STEP buffer</a></li> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/lim">Low Iron Medium (LIM)</a></li> | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/yeast-trafo">Yeast transformation</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/yeast-trafo">Yeast transformation</a></li> | ||
</ul></p> | </ul></p> | ||
Revision as of 19:39, 4 October 2013
Protocols
General and E. coli protocols
- SOB medium
- LB medium
- TAE buffer
- LB plates
- DNA-ladder-buffer mix
- Gelelectrophoresis
- Inoue buffer
- Chemo-competent E. coli cells
- E. coli transformation
- Ligation of digested parts and vectors
- Glycerol stocks
- PCR
- 3A-Assembly
- Preparative restriction digest
- Control restriction digest
- Mini Prep
- Gel Extraction
- Colony-PCR
- Handling of the Tecan reader
Saccharomyces cerevisiae-specific protocols
