Team:Freiburg/Highlights
From 2013.igem.org
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dCas9 - The Heart of our toolkit | dCas9 - The Heart of our toolkit | ||
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- | <p>We started by <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/1#introduction">mutating</a> the DNA cleavage site in the Cas9 protein and therefore generated a DNA binding protein. The binding mechanism is relying on a protein-RNA-DNA interaction. By changing the RNA sequence we are now able to determine the DNA binding locus and can direct the protein to any requested DNA target.</p> | + | <p>We started by <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/1#introduction">mutating</a> the DNA cleavage site in the Cas9 protein and therefore generated a DNA binding protein. The binding mechanism is relying on a protein-RNA-DNA interaction. By changing the RNA sequence we are now able to determine the DNA binding locus and can direct the protein to any requested DNA target. <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/1#introduction">Read more!</a></p> |
<p>This is the <b>heart of our toolkit</b>. A protein that allows multiple and sequence specified DNA binding. By fusing effector domains to dCas9 we created fusion proteins with completely new function. Read more in the next slides.</p> | <p>This is the <b>heart of our toolkit</b>. A protein that allows multiple and sequence specified DNA binding. By fusing effector domains to dCas9 we created fusion proteins with completely new function. Read more in the next slides.</p> | ||
Revision as of 20:06, 4 October 2013
HIGHLIGHTS