Team:Freiburg/Highlights
From 2013.igem.org
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dCas9 - The heart of our toolkit | dCas9 - The heart of our toolkit | ||
</p> | </p> | ||
- | <p>The CRISPR/Cas9 system relies on a protein-RNA-DNA interaction between the Cas9 protein and | + | <p>The CRISPR/Cas9 system relies on a protein-RNA-DNA interaction between the Cas9 protein and two non-coding RNAs. The |
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160 kDa large Cas9 protein was <a id="link" href="https://2013.igem.org/Team:Freiburg/Notebook/standardisation">mutated</a> | 160 kDa large Cas9 protein was <a id="link" href="https://2013.igem.org/Team:Freiburg/Notebook/standardisation">mutated</a> | ||
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by inactivating the DNA cleavage site and standardized for the iGEM community by introducing 10 mutations into the | by inactivating the DNA cleavage site and standardized for the iGEM community by introducing 10 mutations into the | ||
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<i>cas9</i> gene resulting in the DNA-binding protein dCas9 found in the parts registry (<a id="link" | <i>cas9</i> gene resulting in the DNA-binding protein dCas9 found in the parts registry (<a id="link" | ||
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href="http://parts.igem.org/Part:BBa_K1150000">BBa_K1150000</a>).</p> | href="http://parts.igem.org/Part:BBa_K1150000">BBa_K1150000</a>).</p> | ||
- | <p>This is the heart of our toolkit: | + | <p>This is the heart of our toolkit: A protein that allows for multiple and sequence-specific DNA binding. By |
- | + | fusing various effector domains fused to dCas9, we constructed novel proteins allowing for efficient gene regulation. Read more in the next slides.</p> | |
- | fusing various effector domains to dCas9, we constructed novel proteins allowing for efficient gene regulation. Read more | + | |
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- | in the next slides.</p> | + | |
Revision as of 21:09, 4 October 2013
HIGHLIGHTS