Team:TU-Eindhoven/Achievements
From 2013.igem.org
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Below stands the judging criteria we comply with. | Below stands the judging criteria we comply with. | ||
- | {{:Team:TU-Eindhoven/Template:FloatingImage | position=left | size=1 | filename=bronzeMedal.png}} ==Bronze Medal== | + | {{:Team:TU-Eindhoven/Template:FloatingImage | position=left | size=1 | filename=bronzeMedal.png}} |
- | + | == Bronze Medal == | |
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<html><i class="icon-ok"></i></html> Team registration. | <html><i class="icon-ok"></i></html> Team registration. |
Revision as of 00:40, 5 October 2013
![](https://static.igem.org/mediawiki/2013/6/6d/TU-Eindhoven_Images_LogoTransp.png)
![](https://static.igem.org/mediawiki/2013/3/32/TU-Eindhoven_Images_MRiGEM.png)
![](http://2013.igem.org/wiki/images/4/45/TU-Eindhoven_iGEM_small.png)
Our Achievements
Below stands the judging criteria we comply with.
![TU-Eindhoven Images bronzeMedal.png](/wiki/images/e/e5/TU-Eindhoven_Images_bronzeMedal.png)
Bronze Medal
Team registration.
Complete Judging form.
Team's project description on the wiki and team's part on the Registry of Standard Biological Parts.
Present a poster and talk at the iGEM Jamboree.
At least one new standard BioBrick Part or Device:
- From BBa_K1123000 to BBa_K1123021
![TU-Eindhoven Images silverMedal.png](/wiki/images/a/a8/TU-Eindhoven_Images_silverMedal.png)
![TU-Eindhoven Images checkmark.png](/wiki/images/0/0c/TU-Eindhoven_Images_checkmark.png)
![TU-Eindhoven Images checkmark.png](/wiki/images/0/0c/TU-Eindhoven_Images_checkmark.png)
- BBa_K1123003 and BBa_K1123005
![TU-Eindhoven Images checkmark.png](/wiki/images/0/0c/TU-Eindhoven_Images_checkmark.png)
These aspects have being considered on our project and the detail description can be found on the public safety section of our wiki.
![TU-Eindhoven Images goldMedal.png](/wiki/images/8/88/TU-Eindhoven_Images_goldMedal.png)
![TU-Eindhoven Images checkmark.png](/wiki/images/0/0c/TU-Eindhoven_Images_checkmark.png)
For the 2013 TU-Eindhoven iGEM project we wished to express MRI contrast providing proteins upon bacteria entering anaerobic tumours. To induce the expression of these proteins we would be making use of an FNR promoter just like the NirB promoter. To design our promoter we made use of an article (Anne M.L. Barnard, Jeffrey Green, and Stephen J.W. Busby, Transcription regulation by tandem-bound FNR at Escherichia coli promoters. Journal of bacteriology 185.20, 5993-6004 (2003)). From this article we extracted the data for our promoter, by choosing the most optimal promoter given in the articles selection.
To test the efficiency of this promoter we, much like the Valencia 2012 team, expressed EGFP under anaerobic conditions in which, again like the Valencia 2012 team were successful. Based on the research by Barnard and al. and our measurements, Part: BBa_K1123005 seems to have more potential as a FNR promoter with higher activity.
![TU-Eindhoven Images checkmark.png](/wiki/images/0/0c/TU-Eindhoven_Images_checkmark.png)
- Purdue iGEM Team: we participated in the improvement of the current characterization forms.
- Copenhagen iGEM Team: we participated on the Bricks of Knowledge initiative by making a video about creating an anaerobic environment.
![TU-Eindhoven Images checkmark.png](/wiki/images/0/0c/TU-Eindhoven_Images_checkmark.png)
We developed an online system Synthetic Facts to provide the general public with unbiased trustworthy knowledge about synthetic biology.