Team:BYU Provo/Notebook/Cholera - Enzyme/October/Period1/Dailylog

10/1/13 - 10/3/13

We spent these days finalizing our presentation and poster with the data that we had gathered, and left for the North American Jamboree.

10/4/13 - 10/6/13

The North American iGem Jamboree! We had a great time, learned a lot from the other teams, and are excited to be moving on to the World Championships! Now it's time to get back to work and get more data.

10/9/13

We made a new 500 mL stock of our SSW-LB following the recipe listed on the protocols page. We also set up overnights of PIG92 in LB+AMP, so that it can be purified and placed in our parts registry. We also started new cholera overnights from our plated cholera.

10/10/13

We checked the PIG92 overnight that was started yesterday, but it was still clear. We started three more overnights of PIG92 in LB, LB+AMP, and LB+CAM. We will check tomorrow to see which of these is growing correctly.

10/11/13

The overnight of PIG92 that was started on 10/9 is nice and cloudy today, indicating growth of our bacteria. The overnights started yesterday are still clear, including the overnight in LB, so the bacteria just takes a little more time to grow up than we were expecting. We pelleted the overnight from 10/9 and placed it in the freezer for tomorrow.

Our cholera overnights don't show any growth yet, but as we are starting these overnights from plated cholera, it will take almost a week before we have good biofilm growth.

The SSW-LB that we prepared on 10/9 doesn't look right. It is darker than it should be and is really cloudy. We discarded this stock and made a new 500 mL stock following the recipe listed in the protocols section. The new stock looks right and is free of contamination.

Our DspB colony plate grew well, so we took several individual colonies and

10/12/13